ArticlePloS one2010
Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 100 citations in OpenAlex.
- miRNA Sequencing and Differential Analysis of Testis in 1-Year-Old and 2-Year-Old Kazakh Horses.Biology · 2026Article
- Sperm abnormality: Differential expression of microRNAs.Journal of assisted reproduction and genetics · 2025Review
- Comprehensive Analysis of Methylome and Transcriptome to Identify Potential Genes Regulating Porcine Testis Development.International journal of molecular sciences · 2024Article
- Effects of Slow Freezing and Vitrification of Human Semen on Post-Thaw Semen Quality and miRNA Expression.International journal of molecular sciences · 2024Article
- In Silico Analysis of miRNA-Mediated Genes in the Regulation of Dog Testes Development from Immature to Adult Form.Animals : an open access journal from MDPI · 2023Article
- A Comprehensive Sequencing Analysis of Testis-Born miRNAs in Immature and Mature Indigenous Wandong Cattle (Genes · 2022Article
- Correlation between Sperm Micro Ribonucleic Acid-34b and -34c Levels and Clinical Outcomes of Intracytoplasmic Sperm Injection in Men with Male Factor Infertility.International journal of molecular sciences · 2022Article
- Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis.Cellular and molecular life sciences : CMLS · 2022Article
- Follicle-stimulating hormone-mediated decline in miR-92a-3p expression in pubertal mice Sertoli cells is crucial for germ cell differentiation and fertility.Cellular and molecular life sciences : CMLS · 2022Article
- Regulation of mammalian spermatogenesis by miRNAs.Seminars in cell & developmental biology · 2022Review
- Declining levels of miR-382-3p at puberty trigger the onset of spermatogenesis.Molecular therapy. Nucleic acids · 2021Article
- Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars.BMC genomics · 2020Article
- The Transcriptome of Pig Spermatozoa, and Its Role in Fertility.International journal of molecular sciences · 2020Article
- Profiling of miRNAs in porcine Sertoli cells.Journal of animal science and biotechnology · 2020Article
- Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri-implantation embryos.Biological reviews of the Cambridge Philosophical Society · 2019Review
- MicroRNA-322 Regulates Self-renewal of Mouse Spermatogonial Stem Cells throughInternational journal of biological sciences · 2019Article
- MicroRNA Alternations in the Testes Related to the Sterility of Triploid Fish.Marine biotechnology (New York, N.Y.) · 2018Article
- MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins.Nucleic acids research · 2017Article
- Reduced microRNA-188-3p expression contributes to apoptosis of spermatogenic cells in patients with azoospermia.Cell proliferation · 2017Article
- miR-638 Inhibits immature Sertoli cell growth by indirectly inactivating PI3K/AKT pathway via SPAG1 gene.Cell cycle (Georgetown, Tex.) · 2017Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood. METHODOLOGY: We employed a miRNA microarray containing 1260 unique miRNA probes to evaluate the miRNA expression patterns between sexually immature (60-day) and mature (180-day) pig testes. One hundred and twenty nine miRNAs representing 164 reporter miRNAs were expressed differently (p<0.1). Fifty one miRNAs were significantly up-regulated and 78 miRNAs were down-regulated in mature testes. Nine of these differentially expressed miRNAs were validated using quantitative RT-PCR assay. Totally 15,919 putative miRNA-target sites were detected by using RNA22 method to align 445 NCBI pig cDNA sequences with these 129 differentially expressed miRNAs, and seven putative target genes involved in spermatogenesis including DAZL, RNF4 gene were simply confirmed by quantitative RT-PCR.
conclusionsOverall, the results of this study indicated specific miRNAs expression in porcine testes and suggested that miRNAs had a role in regulating spermatogenesis.
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