ReviewNucleic acids research2011
Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity.
Review in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed.
- Review
- Functions and applications of enzymes in nucleic acid nanotechnology.Journal of nanobiotechnology · 2025Review
- Crystal structures of monomeric BsmI restriction endonuclease reveal coordinated sequential cleavage of two DNA strands.Communications biology · 2025Article
- Lack of Association Between BsmI and FokI Polymorphisms of the VDR Gene and Sporadic Colorectal Cancer in a Romanian Cohort-A Preliminary Study.Current oncology (Toronto, Ont.) · 2024Article
- Review
- Biophysical and structural characterization of a multifunctional viral genome packaging motor.Nucleic acids research · 2024Article
- Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses.Analytica chimica acta · 2022Review
- Types and Applications of Nicking Enzyme-Combined Isothermal Amplification.International journal of molecular sciences · 2022Review
- The Influence of 5'Molecules (Basel, Switzerland) · 2021Article
- Engineering Infrequent DNA Nicking Endonuclease by Fusion of aFrontiers in microbiology · 2021Article
- Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage.Nucleic acids research · 2020Article
- A localized molecular automaton forChemical science · 2020Article
- Electrochemiluminescent determination of the activity of uracil-DNA glycosylase: Combining nicking enzyme assisted signal amplification and catalyzed hairpin assembly.Mikrochimica acta · 2019Article
- Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy.Journal of visualized experiments : JoVE · 2018Article
- Structure and dynamics of mesophilic variants from the homing endonuclease I-DmoI.Journal of computer-aided molecular design · 2017Article
- Physical and Functional Characterization of a Viral Genome Maturation Complex.Biophysical journal · 2017Article
- Plasmid-based one-pot saturation mutagenesis.Nature methods · 2016Article
- Mapping the nicking efficiencies of nickase R.BbvCI for side-specific LNA-substituted substrates using rolling circle amplification.Scientific reports · 2016Article
- The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair.DNA repair · 2016Review
- Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Restriction endonucleases (REases) are highly specific DNA scissors that have facilitated the development of modern molecular biology. Intensive studies of double strand (ds) cleavage activity of Type IIP REases, which recognize 4-8 bp palindromic sequences, have revealed a variety of mechanisms of molecular recognition and catalysis. Less well-studied are REases which cleave only one of the strands of dsDNA, creating a nick instead of a ds break. Naturally occurring nicking endonucleases (NEases) range from frequent cutters such as Nt.CviPII (^CCD; ^ denotes the cleavage site) to rare-cutting homing endonucleases (HEases) such as I-HmuI. In addition to these bona fida NEases, individual subunits of some heterodimeric Type IIS REases have recently been shown to be natural NEases. The discovery and characterization of more REases that recognize asymmetric sequences, particularly Types IIS and IIA REases, has revealed recognition and cleavage mechanisms drastically different from the canonical Type IIP mechanisms, and has allowed researchers to engineer highly strand-specific NEases. Monomeric LAGLIDADG HEases use two separate catalytic sites for cleavage. Exploitation of this characteristic has also resulted in useful nicking HEases. This review aims at providing an overview of the cleavage mechanisms of Types IIS and IIA REases and LAGLIDADG HEases, the engineering of their nicking variants, and the applications of NEases and nicking HEases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.