ArticleNucleic acids research2011
Highly redundant function of multiple AT-rich sequences as core promoter elements in the TATA-less RPS5 promoter of Saccharomyces cerevisiae.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Overexpression ofInternational journal of molecular sciences · 2026Article
- Article
- A natural allele ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae.Nature communications · 2025Article
- Article
- Development of Terminator-Promoter Bifunctional Elements for Application inInternational journal of molecular sciences · 2023Article
- Design of an artificial transcriptional system for production of high levels of recombinant proteins in tobacco (Frontiers in plant science · 2023Article
- Controlling gene expression with deep generative design of regulatory DNA.Nature communications · 2022Article
- Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study.Biotechnology for biofuels · 2021Article
- The TAFs of TFIID Bind and Rearrange the Topology of the TATA-LessInternational journal of molecular sciences · 2019Article
- Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species.ACS synthetic biology · 2017Article
- Article
- Core promoter sequence in yeast is a major determinant of expression level.Genome research · 2015Article
- Article
- Mutations on the DNA binding surface of TBP discriminate between yeast TATA and TATA-less gene transcription.Molecular and cellular biology · 2014Article
- Sequence features of yeast and human core promoters that are predictive of maximal promoter activity.Nucleic acids research · 2013Article
- Article
- Review
- DNA shape, genetic codes, and evolution.Current opinion in structural biology · 2011Review
- Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.Nucleic acids research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotes, protein-coding genes are transcribed by RNA polymerase II (pol II) together with general transcription factors (GTFs). TFIID, the largest GTF composed of TATA element-binding protein (TBP) and 14 TBP-associated factors (TAFs), plays a critical role in transcription from TATA-less promoters. In metazoans, several core promoter elements other than the TATA element are thought to be recognition sites for TFIID. However, it is unclear whether functionally homologous elements also exist in TATA-less promoters in Saccharomyces cerevisiae. Here, we identify the cis-elements required to support normal levels of transcription and accurate initiation from sites within the TATA-less and TFIID-dependent RPS5 core promoter. Systematic mutational analyses show that multiple AT-rich sequences are required for these activities and appear to function as recognition sites for TFIID. A single copy of these sequences can support accurate initiation from the endogenous promoter, indicating that they carry highly redundant functions. These results show a novel architecture of yeast TATA-less promoters and support a model in which pol II scans DNA downstream from a recruited site, while searching for appropriate initiation site(s).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.