Evidence map›Paper›PMID 20803555›Full record

ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2011

CB2 cannabinoid receptor targets mitogenic Gi protein-cyclin D1 axis in osteoblasts.

Orr Ofek, Malka Attar-Namdar, Vardit Kram, Mona Dvir-Ginzberg, Raphael Mechoulam, Andreas Zimmer, Baruch Frenkel, Esther Shohami, Itai Bab

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.

  1. Effect of recreational cannabis use on bone mineral density: a systematic review.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2024
    Pooled it
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  6. Cannabidiol and Cannabigerol, Nonpsychotropic Cannabinoids, as Analgesics that Effectively Manage Bone Fracture Pain and Promote Healing in Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023
    Article
  7. Review
  8. Article
  9. HU308 Mitigates Osteoarthritis by Stimulating Sox9-Related Networks of Carbohydrate Metabolism.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023
    Article
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  13. Article
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  19. The Endocannabinoid System in Pediatric Inflammatory and Immune Diseases.International journal of molecular sciences · 2019
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Orr OfekBone Laboratory, Hebrew University of Jerusalem, Jerusalem, Israel.
Malka Attar-Namdar
Vardit Kram
Mona Dvir-Ginzberg
Raphael Mechoulam
Andreas Zimmer
Baruch Frenkel
Esther Shohami
Itai Bab
Hebrew University of Jerusalem · ILUniversity of Bonn · DEUniversity of Southern California · US

Funding

REGULATION OF CHROMATINIZED GENES IN OSTEOBLASTSR01AR047052 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FRENKEL, BARUCH · 2000 to 2010
$2.8M
NIAMS NIH HHS AR047052NIAMS NIH HHS R01 AR047052PHS HHS 9789
6 · The paper itself

Abstract

CB2 is a Gi protein-coupled receptor activated by endo- and phytocannabinoids, thus inhibiting stimulated adenylyl cyclase activity. CB2 is expressed in bone cells and Cb2 null mice show a marked age-related bone loss. CB2-specific agonists both attenuate and rescue ovariectomy-induced bone loss. Activation of CB2 stimulates osteoblast proliferation and bone marrow derived colony-forming units osteoblastic. Here we show that selective and nonselective CB2 agonists are mitogenic in MC3T3 E1 and newborn mouse calvarial osteoblastic cultures. The CB2 mitogenic signaling depends critically on the stimulation of Erk1/2 phosphorylation and de novo synthesis of MAP kinase-activated protein kinase 2 (Mapkapk2) mRNA and protein. Further downstream, CB2 activation enhances CREB transcriptional activity and cyclin D1 mRNA expression. The CB2-induced stimulation of CREB and cyclin D1 is inhibitable by pertussis toxin, the MEK-Erk1/2 inhibitors PD098059 and U0126, and Mapkapk2 siRNA. These data demonstrate that in osteoblasts CB2 targets a Gi protein-cyclin D1 mitogenic axis. Erk1/2 phosphorylation and Mapkapk2 protein synthesis are critical intermediates in this axis.

Indexed as

3T3 CellsAnimalsButadienesCyclin D1FlavonoidsGTP-Binding Protein alpha Subunits, Gi-GoMiceMice, Inbred C57BLMice, TransgenicMitogensNitrilesOsteoblastsReceptor, Cannabinoid, CB2RNA, MessengerRNA, Small Interfering2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-oneButadienesCyclin D1FlavonoidsGTP-Binding Protein alpha Subunits, Gi-GoMitogensNitrilesReceptor, Cannabinoid, CB2RNA, MessengerRNA, Small InterferingU 0126

Identifiers

PMID20803555
PMCPMC3179350
OpenAlexW2010079727

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.