ArticleJournal of cellular biochemistry2010
Optimizing stem cell culture.
Article in Journal of cellular biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Advancing cell therapies with artificial intelligence and synthetic biology.Current opinion in biomedical engineering · 2025Article
- Droplet microarray platforms for high-throughput drug screening.Mikrochimica acta · 2023Review
- Favorable culture conditions for spermatogonial propagation in human and non-human primate primary testicular cell cultures: a systematic review and meta-analysis.Frontiers in cell and developmental biology · 2023Article
- Insight and Recent Advances into the Role of Topography on the Cell Differentiation and Proliferation on Biopolymeric Surfaces.International journal of molecular sciences · 2022Review
- Revisiting Epithelial Carcinogenesis.International journal of molecular sciences · 2022Article
- In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture.Communications biology · 2022Article
- Applications of 3D Bioprinting Technology in Induced Pluripotent Stem Cells-Based Tissue Engineering.Micromachines · 2022Review
- Bioprinting of Complex Multicellular Organs with Advanced Functionality-Recent Progress and Challenges Ahead.Advanced materials (Deerfield Beach, Fla.) · 2022Review
- Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application-A Review.Polymers · 2021Review
- Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit.SLAS technology · 2021Article
- Optimizing theJournal of stem cells & regenerative medicine · 2020Article
- Silk Polymers and Nanoparticles: A Powerful Combination for the Design of Versatile Biomaterials.Frontiers in chemistry · 2020Review
- Cell Survival Effects of Autophagy Regulation on Umbilical Cord-Derived Mesenchymal Stem Cells Following Exposure to Oxidative Stress.Iranian journal of medical sciences · 2019Article
- Automated sensing and splitting of stem cell colonies on microraft arrays.APL bioengineering · 2019Article
- Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential.PloS one · 2019Article
- Modeling Mammalian Commitment to the Neural Lineage Using Embryos and Embryonic Stem Cells.Frontiers in physiology · 2019Review
- Neural stem cells deriving from chick embryonic hindbrain recapitulate hindbrain development in culture.Scientific reports · 2018Article
- Regulation of the fate of dental-derived mesenchymal stem cells using engineered alginate-GelMA hydrogels.Journal of biomedical materials research. Part A · 2017Article
- Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice.Cell adhesion & migration · 2017Article
- Biomimicry in mending the broken heart; Will hypoxia and pulsatile flow play Cupids?Journal of stem cells & regenerative medicine · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells always balance between self-renewal and differentiation. Hence, stem cell culture parameters are critical and need to be continuously refined according to progress in our stem cell biology understanding and the latest technological developments. In the past few years, major efforts have been made to define more precisely the medium composition in which stem cells grow or differentiate. This led to the progressive replacement of ill-defined additives such as serum or feeder cell layers by recombinant cytokines or growth factors. Another example is the control of the oxygen pressure. For many years cell cultures have been done under atmospheric oxygen pressure which is much higher than the one experienced by stem cells in vivo. A consequence of cell metabolism is that cell culture conditions are constantly changing. Therefore, the development of high sensitive monitoring processes and control algorithms is required for ensuring cell culture medium homeostasis. Stem cells also sense the physical constraints of their microenvironment. Rigidity, stiffness, and geometry of the culture substrate influence stem cell fate. Hence, nanotopography is probably as important as medium formulation in the optimization of stem cell culture conditions. Recent advances include the development of synthetic bioinformative substrates designed at the micro- and nanoscale level. On going research in many different fields including stem cell biology, nanotechnology, and bioengineering suggest that our current way to culture cells in Petri dish or flasks will soon be outdated as flying across the Atlantic Ocean in the Lindbergh's plane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.