ArticleAmerican journal of physiology. Heart and circulatory physiology2010
cGMP-dependent protein kinase and the regulation of vascular smooth muscle cell gene expression: possible involvement of Elk-1 sumoylation.
Article in American journal of physiology. Heart and circulatory physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- The Phosphoproteome of the Rd1 Mouse Retina, a Model of Inherited Photoreceptor Degeneration, Changes after Protein Kinase G Inhibition.International journal of molecular sciences · 2023Article
- Intrauterine Nitric Oxide Deficiency Weakens Differentiation of Vascular Smooth Muscle in Newborn Rats.International journal of molecular sciences · 2021Article
- SUMO2 regulates vascular endothelial function and oxidative stress in mice.American journal of physiology. Heart and circulatory physiology · 2019Article
- cGMP Signaling and Vascular Smooth Muscle Cell Plasticity.Journal of cardiovascular development and disease · 2018Review
- Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation.Physiological genomics · 2017Article
- Vascular smooth muscle cell contractile protein expression is increased through protein kinase G-dependent and -independent pathways by glucose-6-phosphate dehydrogenase inhibition and deficiency.American journal of physiology. Heart and circulatory physiology · 2016Article
- 20-HETE-induced mitochondrial superoxide production and inflammatory phenotype in vascular smooth muscle is prevented by glucose-6-phosphate dehydrogenase inhibition.American journal of physiology. Heart and circulatory physiology · 2016Article
- Heparin responses in vascular smooth muscle cells involve cGMP-dependent protein kinase (PKG).Journal of cellular physiology · 2014Article
- Contractile protein expression is upregulated by reactive oxygen species in aorta of Goto-Kakizaki rat.American journal of physiology. Heart and circulatory physiology · 2014Article
- Hypoxic depression of PKG-mediated inhibition of serotonergic contraction in ovine carotid arteries.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Transcriptional and post-transcriptional regulation of cGMP-dependent protein kinase (PKG-I): pathophysiological significance.Cardiovascular research · 2013Review
- Relationships between vascular oxygen sensing mechanisms and hypertensive disease processes.Hypertension (Dallas, Tex. : 1979) · 2012Review
- Glc-6-PD and PKG contribute to hypoxia-induced decrease in smooth muscle cell contractile phenotype proteins in pulmonary artery.American journal of physiology. Lung cellular and molecular physiology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Although the regulation of smooth muscle cell (SMC) gene expression by cGMP-dependent protein kinase (PKG) is now recognized, the mechanisms underlying these effects are not fully understood. In this study, we report that PKG-I stimulates myocardin/serum response factor (SRF)-dependent gene expression in vascular SMCs. The expression of PKG in PKG-deficient cells enhanced myocardin-induced SM22 promoter activity in a concentration-dependent fashion. However, neither SRF nor myocardin expression was affected. To investigate alternative mechanisms, we examined whether PKG affects the phosphorylation of E26-like protein-1 (Elk-1), a SRF/myocardin transcription antagonist. The activation of PKG caused an increase in a higher molecular mass form of phospho-Elk-1 that was determined to be small ubiquitin-related modifier (sumo)ylated Elk-1. PKG increased Elk-1 sumoylation twofold compared with the PKG-deficient cells, and Elk-1 sumoylation was reduced using dominant-negative sumo-conjugating enzyme, DN-Ubc9, confirming PKG-dependent sumoylation of phospho-Elk-1 in vascular SMCs. In addition, PKG stimulated Elk-1 sumoylation in COS-7 cells overexpressing Elk-1, sumo-1, and PKG-I. The increased expression of PKG in vascular SMCs inhibited Elk-1 binding to SMC-specific promoters, SM22 and smooth muscle myosin heavy chain, as measured by EMSA and chromatin immunoprecipitation assay, and PKG suppressed the Elk-1 inhibition of SM22 reporter gene expression. Taken together, these data suggest that PKG-I decreases Elk-1 activity by sumo modification of Elk-1, thereby increasing myocardin-SRF activity on SMC-specific gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.