Evidence map›Paper›PMID 20802067›Full record

ArticleApplied and environmental microbiology2010

Enhancement of targeted homologous recombination in Mycoplasma mycoides subsp. capri by inclusion of heterologous recA.

Ayman B Allam, Leticia Reyes, Nacyra Assad-Garcia, John I Glass, Mary B Brown

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.2field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 20 citations in OpenAlex.

  1. Unravelling Antimicrobial Resistance inVeterinary sciences · 2024
    Review
  2. Review
  3. Article
  4. TheFrontiers in microbiology · 2022
    Review
  5. Construction ofFrontiers in microbiology · 2021
    Article
  6. Review
  7. ACS synthetic biology · 2020
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ayman B AllamDepartment of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Leticia Reyes
Nacyra Assad-Garcia
John I Glass
Mary B Brown
University of Florida · USJ. Craig Venter Institute · US

Funding

TRAINING PROGRAM IN HUMAN NUTRITION AND METABOLISMT32DK007651 · NIDDK · TUFTS UNIVERSITY BOSTON · PI MASON, JOEL B · 1990 to 2009
$1.9M
NIDDK NIH HHS 5K08DK07651NIDDK NIH HHS T32 DK007651
6 · The paper itself

Abstract

A suicide plasmid, pExp1-ctpA::tetM-recAec, employing recA from Escherichia coli and tetM as a selection marker, was used to generate ctpA knockout mutants in Mycoplasma mycoides subsp. capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both the consistency and the recovery of mutants generated by homologous recombination.

Indexed as

Recombination, GeneticAlgal ProteinsBacterial ProteinsCarboxypeptidasesEscherichia coliGene Knockout TechniquesGene TargetingGenetic VectorsMycoplasma mycoidesPlasmidsProprotein ConvertasesRec A RecombinasesRecombinant ProteinsSelection, GeneticTetracycline ResistanceAlgal ProteinsBacterial ProteinsCarboxypeptidasesphotosystem II, D1 polypeptide carboxy-terminal processing proteaseProprotein ConvertasesRec A RecombinasesRecombinant ProteinsTet M resistance protein, Bacteria

Identifiers

PMID20802067
PMCPMC2953016
OpenAlexW2038211252

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.