ArticleBiochimica et biophysica acta2010
Hepcidin expression by human monocytes in response to adhesion and pro-inflammatory cytokines.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01726400 (Is Hepcidin a Possible Contributor to Impaired Iron Mobilization and Anaemia in Hepatitis C Patients Treated With Pegylated Interferon Alpha and Ribavirin Therapy? A Pilot Study), which is not on this map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Is Hepcidin a Possible Contributor to Impaired Iron Mobilization and Anaemia in Hepatitis C Patients Treated With Pegylated Interferon Alpha and Ribavirin Therapy? A Pilot Study
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Biomarkers of systemic lupus erythematosus identified using mass spectrometry-based proteomics: a systematic review.Journal of cellular and molecular medicine · 2017Pooled it
- Maternal Adiposity and Inflammation: Risk Factors for Iron Deficiency in Pregnancy.The Journal of nutrition · 2025Trial
- Tubular reabsorption and local production of urine hepcidin-25.BMC nephrology · 2013Trial
- Association of the monocyte-to-lymphocyte ratio with HIV viral load and anemia in HIV-positive patients: a retrospective cross-sectional study.Frontiers in cellular and infection microbiology · 2026Article
- Serum Hepcidin Levels are Associated with Poor Outcomes of Patients with Severe Traumatic Brain Injury: A Two-Center Observational Analytical Study.International journal of general medicine · 2026Article
- Enterolignans Improve the Expression of Iron-Related Genes in a Cellular Model of Inflammatory Bowel Disease.International journal of molecular sciences · 2025Article
- Article
- Distinctive modulation of hepcidin in cancer and its therapeutic relevance.Frontiers in oncology · 2023Review
- Cellular stress responses and dysfunctional Mitochondrial-cellular senescence, and therapeutics in chronic respiratory diseases.Redox biology · 2020Review
- Importance of Studying the Levels of Hepcidin and Vitamin D in Egyptian Children with Chronic Hepatitis C.Journal of translational internal medicine · 2019Article
- Serum Concentration of Hepcidin as an Indicator of Iron Reserves in Children.Journal of medical biochemistry · 2018Article
- MyD88 Regulates the Expression of SMAD4 and the Iron Regulatory Hormone Hepcidin.Frontiers in cell and developmental biology · 2018Article
- Biomarkers of inflammation and innate immunity in atrophic nonunion fracture.Journal of translational medicine · 2016Article
- Laboratory use of hepcidin in renal transplant recipients.Biochemia medica · 2016Review
- The pathogenesis of cardiomyopathy in Friedreich ataxia.PloS one · 2015Article
- A study of hepcidin and monocyte chemoattractant protein-1 in Egyptian females with systemic lupus erythematosus.Journal of clinical laboratory analysis · 2014Article
- Serum hepcidin levels are associated with obesity but not liver disease.Obesity (Silver Spring, Md.) · 2014Article
- Hepcidin induction by pathogens and pathogen-derived molecules is strongly dependent on interleukin-6.Infection and immunity · 2014Article
- Rhodomyrtone modulates innate immune responses of THP-1 monocytes to assist in clearing methicillin-resistant Staphylococcus aureus.PloS one · 2014Article
- Biomarkers and updates on pediatrics lupus nephritis.Rheumatic diseases clinics of North America · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundA previous urine proteomic analysis from our laboratory suggested that hepcidin may be a biomarker for lupus nephritis flare. Immunohistochemical staining of kidney biopsies from lupus patients showed that hepcidin was expressed by infiltrating renal leukocytes. Here we investigated whether inflammatory cytokines relevant to the pathogenesis of lupus nephritis and other glomerular diseases regulate hepcidin expression by human monocytes.
methodsHuman CD14+ monocytes were incubated with interferon alpha (IFNα), interferon gamma (IFNγ), interleukin-6 (IL6), interleukin-1 beta (IL1β), monocyte chemotactic factor-1 (MCP1), or tumor necrosis factor alpha (TNFα). Hepcidin expression was examined by real-time PCR and enzyme immunoassay.
resultsMonocyte hepcidin mRNA increased during adherence to the tissue culture wells, reaching a level 150-fold higher than baseline within 12 h of plating. After accounting for the effects of adhesion, monocytes showed time and dose-dependent up-regulation of hepcidin mRNA upon treatment with IFNα or IL6. One hour of incubation with IFNα or IL6 increased hepcidin mRNA 20 and 80-fold, respectively; by 24 h the mRNA remained 5- and 2.4-fold higher than baseline. IL1β, IFNγ, and MCP-1 did not affect monocyte hepcidin expression. TNFα inhibited hepcidin induction by IL6 in monocytes by 44%. After 24 h of treatment with IFNα or IL6, immunoreactive hepcidin production by monocytes increased 3- and 2.6-fold, respectively.
conclusionHuman monocytes produce hepcidin in response to adhesion and the pro-inflammatory cytokines IFNα and IL6. GENERAL SIGNIFICANCE: The appearance of hepcidin in the kidneys or urine during glomerular diseases may be from infiltrating monocytes induced to express hepcidin by adherence and exposure to pro-inflammatory cytokines found in the renal milieu.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.