ArticleUltrasound in medicine & biology2010
Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery.
Article in Ultrasound in medicine & biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Development and mechanism exploration of a quantitative model for Escherichia coli transformation efficiency based on ultrasonic power.Ultrasonics sonochemistry · 2024Article
- Mesenchymal stem cell cryopreservation with cavitation-mediated trehalose treatment.Communications engineering · 2024Article
- The promising shadow of microbubble over medical sciences: from fighting wide scope of prevalence disease to cancer eradication.Journal of biomedical science · 2021Review
- Ultrasound-Mediated Drug Delivery With a Clinical Ultrasound System:Frontiers in pharmacology · 2021Article
- Encapsulation and Ultrasound-Triggered Release of G-Quadruplex DNA in Multilayer Hydrogel Microcapsules.Polymers · 2018Article
- The present and future role of ultrasound targeted microbubble destruction in preclinical studies of cardiac gene therapy.Journal of thoracic disease · 2018Review
- Ultrasound-Triggered Effects of the Microbubbles Coupled to GDNF Plasmid-Loaded PEGylated Liposomes in a Rat Model of Parkinson's Disease.Frontiers in neuroscience · 2018Article
- Transfection of neurotrophin-3 into neural stem cells using ultrasound with microbubbles to treat denervated muscle atrophy.Experimental and therapeutic medicine · 2018Article
- Evaluation of in vivo antitumor effects of low-frequency ultrasound-mediated miRNA-133a microbubble delivery in breast cancer.Cancer medicine · 2016Article
- Novel delivery approaches for cancer therapeutics.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Reducing Neointima Formation in a Swine Model with IVUS and Sirolimus Microbubbles.Annals of biomedical engineering · 2015Article
- Article
- Improving the efficacy of therapeutic angiogenesis by UTMD-mediated Ang-1 gene delivery to the infarcted myocardium.International journal of molecular medicine · 2015Article
- Evaluation of in vivo antitumor effects of ANT2 shRNA delivered using PEI and ultrasound with microbubbles.Gene therapy · 2015Article
- Article
- Localized in vivo model drug delivery with intravascular ultrasound and microbubbles.Ultrasound in medicine & biology · 2014Article
- Antibody-based imaging strategies for cancer.Cancer metastasis reviews · 2014Review
- Characterization of the dynamic activities of a population of microbubbles driven by pulsed ultrasound exposure in sonoporation.Ultrasound in medicine & biology · 2014Article
- Mechanisms of microbubble-facilitated sonoporation for drug and gene delivery.Therapeutic delivery · 2014Review
- Cellular characterization of ultrasound-stimulated microbubble radiation enhancement in a prostate cancer xenograft model.Disease models & mechanisms · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We investigated a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles carrying reporter plasmid DNA were acoustically ruptured in the vicinity of smooth muscle cells in vitro under a range of acoustic pressures (0 to 950 kPa) and pulse durations (0 to 100 cycles). No effect on gene transfection or viability was observed from application of microbubbles, DNA or ultrasound alone. Microbubbles in combination with ultrasound (500-kPa, 1-MHz, 50-cycle bursts at a pulse repetition frequency [PRF] of 100 Hz) significantly reduced viability both with DNA (53 +/- 27%) and without (19 +/- 8%). Maximal gene transfection ( approximately 1% of cells) occurred using 50-cycle, 1-MHz pulses at 300 kPa, which resulted in 40% viability of cells. We demonstrated that we can locally deliver DNA to vascular smooth muscle cells in vitro using microbubble carriers and focused ultrasound.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.