ArticleVirology journal2010
Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Myricetin inhibits transmissible gastroenteritis virus replication by targeting papain-like protease deubiquitinating enzyme activity.Frontiers in microbiology · 2024Article
- Development of a triplex quantitative reverse transcription-polymerase chain reaction for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, and porcine rotavirus A.Frontiers in microbiology · 2024Article
- Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip.BMC veterinary research · 2022Article
- Establishment of a porcine parvovirus (PPV) LAMP visual rapid detection method.Journal of virological methods · 2020Article
- Development of a loop-mediated isothermal amplification assay for the detection and quantification of epizootic epitheliotropic disease virus (salmonid herpesvirus-3).Journal of virological methods · 2019Article
- Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP).Scientific reports · 2018Article
- Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification.Journal of virological methods · 2018Article
- Reverse transcription loop-mediated isothermal amplification for rapid and quantitative assay of covert mortality nodavirus in shrimp.Journal of invertebrate pathology · 2017Article
- A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification.Biological research · 2017Article
- Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification.Journal of virological methods · 2015Article
- Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses.Journal of virological methods · 2013Article
- Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1.Virus genes · 2012Article
- Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63.Journal of virological methods · 2011Article
- Specific, simple and rapid detection of porcine circovirus type 2 using the loop-mediated isothermal amplification method.Virology journal · 2011Article
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Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A conserved nucleic acid fragment of the nucleocapsid gene of Swine Transmissible Gastroenteritis Coronavirus (TGEV) was chosen as the target, six special primers were designed successfully. Loop-mediated isothermal amplification (LAMP) was developed to detect the TGEV by incubation at 60°C for 1 h and the product specificity was confirmed by HphI digestion. Standard curves with high accuracy for TGEV quantization was constructed by adding 1 × SYBR greenI in the LAMP reaction. The assay established in this study was found to detect only the TGEV and no cross-reaction with other viruses, demonstrating its high specificity. By using serial sample dilutions as templates, the detection limit of LAMP was about 10 pg RNA, 10 times more sensitive than that of PCR and could be comparable to the nest-PCR.
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Registered trials
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