ArticleRespiratory research2010
Hypertonic saline increases lung epithelial lining fluid glutathione and thiocyanate: two protective CFTR-dependent thiols against oxidative injury.
Article in Respiratory research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Physiology and pathophysiology of mucus and mucolytic use in critically ill patients.Critical care (London, England) · 2025Review
- Mapping the oxidative landscape in cystic fibrosis: methodological frontiers and application.Frontiers in pharmacology · 2025Review
- CFTR dysfunction leads to defective bacterial eradication on cystic fibrosis airways.Frontiers in physiology · 2024Review
- Proteinous Components of Neutrophil Extracellular Traps Are Arrested by the Cell Wall Proteins ofCells · 2021Article
- The effects of neutrophil-generated hypochlorous acid and other hypohalous acids on host and pathogens.Cellular and molecular life sciences : CMLS · 2021Review
- The Role of Thiocyanate in Modulating Myeloperoxidase Activity during Disease.International journal of molecular sciences · 2020Review
- Effects of Ammonium Chloride on Ozone-induced Airway Inflammation: the Role of Slc26a4 in the Lungs of Mice.Journal of Korean medical science · 2020Article
- The thiocyanate analog selenocyanate is a more potent antimicrobial pro-drug that also is selectively detoxified by the host.Free radical biology & medicine · 2020Article
- Functional and metabolic impairment in cigarette smoke-exposed macrophages is tied to oxidative stress.Scientific reports · 2019Article
- Determination of thiocyanate in exhaled breath condensate.Free radical biology & medicine · 2018Article
- Impaired defenses of neonatal mouse alveolar macrophage withPhysiological reports · 2017Article
- Neutrophil plasticity enables the development of pathological microenvironments: implications for cystic fibrosis airway disease.Molecular and cellular pediatrics · 2016Review
- Eosinophil Peroxidase Catalyzed Protein Carbamylation Participates in Asthma.The Journal of biological chemistry · 2016Article
- Repurposing tromethamine as inhaled therapy to treat CF airway disease.JCI insight · 2016Article
- Monitoring the intracellular calcium response to a dynamic hypertonic environment.Scientific reports · 2016Article
- Epithelial Anion Transport as Modulator of Chemokine Signaling.Mediators of inflammation · 2016Review
- Antiinflammatory and Antimicrobial Effects of Thiocyanate in a Cystic Fibrosis Mouse Model.American journal of respiratory cell and molecular biology · 2015Article
- Biochemical mechanisms and therapeutic potential of pseudohalide thiocyanate in human health.Free radical research · 2015Review
- Prevention of acute respiratory distress syndrome.Current opinion in critical care · 2015Review
- Redox balance in cystic fibrosis.The international journal of biochemistry & cell biology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCystic fibrosis is a debilitating lung disease due to mutations in the cystic fibrosis transmembrane conductance regulator protein (CFTR) and is associated with chronic infections resulting in elevated myeloperoxidase activity and generation of hypochlorous acid (HOCl). CFTR mutations lead to decreased levels of glutathione (GSH) and thiocyanate (SCN) in the epithelial lining fluid (ELF). Hypertonic saline is used to improve lung function however the mechanism is uncertain.
methodsIn the present study, the effect of GSH and SCN on HOCl-mediated cell injury and their changes in the ELF after hypertonic saline nebulization in wild type (WT) and CFTR KO mice was examined. CFTR sufficient and deficient lung cells were assessed for GSH, SCN and corresponding sensitivity towards HOCl-mediated injury, in vitro.
resultsCFTR (-) cells had lower extracellular levels of both GSH and SCN and were more sensitive to HOCl-mediated injury. In vivo, hypertonic saline increased ELF GSH in the WT and to a lesser extent in the CFTR KO mice but only SCN in the WT ELF. Finally, potential protective effects of GSH and SCN at concentrations found in the ELF against HOCl toxicity were examined in vitro.
conclusionsWhile the concentrations of GSH and SCN associated with the WT ELF protect against HOCl toxicity, those found in the CFTR KO mice were less sufficient to inhibit cell injury. These data suggests that CFTR has important roles in exporting GSH and SCN which are protective against oxidants and that hypertonic saline treatment may have beneficial effects by increasing their levels in the lung.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.