ArticleJournal of biomedical optics
Three-dimensional optical method for integrated visualization of mouse islet microstructure and vascular network with subcellular-level resolution.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas.Scientific reports · 2024Article
- 3D Optical Reconstruction of the Nervous System of the Whole-Body Marine Invertebrates.Chemical & biomedical imaging · 2023Article
- Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes.Frontiers in endocrinology · 2021Review
- Lipophilic dye-compatible brain clearing technique allowing correlative magnetic resonance/high-resolution fluorescence imaging in rat models of glioblastoma.Scientific reports · 2020Article
- Optical clearing of the pancreas for visualization of mature β-cells and vessels in mice.Islets · 2018Article
- Pancreatic neuro-insular network in young mice revealed by 3D panoramic histology.Diabetologia · 2018Article
- Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow.Biomedical optics express · 2016Article
- PanIN-associated pericyte, glial, and islet remodeling in mice revealed by 3D pancreatic duct lesion histology.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- 3-D imaging of islets in obesity: formation of the islet-duct complex and neurovascular remodeling in young hyperphagic mice.International journal of obesity (2005) · 2016Article
- Optical clearing based cellular-level 3D visualization of intact lymph node cortex.Biomedical optics express · 2015Article
- 3-D Imaging Reveals Participation of Donor Islet Schwann Cells and Pericytes in Islet Transplantation and Graft Neurovascular Regeneration.EBioMedicine · 2015Article
- Feasibility of a hybrid elastographic-microfluidic device to rapidly process and assess pancreatic cancer biopsies for pathologists.... Health innovations and point-of-care technologies conference. Health innovations and point-of-care technologies conference · 2014Article
- Article
- Recent progress in tissue optical clearing.Laser & photonics reviews · 2013Article
- 3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].PloS one · 2013Article
- 3-D neurohistology of transparent tongue in health and injury with optical clearing.Frontiers in neuroanatomy · 2013Article
- Optical histology: a method to visualize microvasculature in thick tissue sections of mouse brain.PloS one · 2013Article
- 3-D imaging and illustration of the perfusive mouse islet sympathetic innervation and its remodelling in injury.Diabetologia · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-based, 2-D tissue analysis confines visualization of the islet architecture at a specific cut plane, 3-D representation of image data is preferable for islet assessment. We applied optical clearing to minimize the random light scattering in the mouse pancreatic tissue. The optical-cleared pancreas allowed penetrative, 3-D microscopic imaging of the islet microstructure and vasculature. Specifically, the islet vasculature was revealed by vessel painting-lipophilic dye labeling of blood vessels-for confocal microscopy. The voxel-based confocal micrographs were digitally processed with projection algorithms for 3-D visualization. Unlike the microtome-based tissue imaging, this optical method for penetrative imaging of mouse islets yielded clear, continuous optical sections for an integrated visualization of the islet microstructure and vasculature with subcellular-level resolution. We thus provide a useful imaging approach to change our conventional planar view of the islet structure into a 3-D panorama for better understanding of the islet physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.