ArticleNucleic acids research2011
Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 36 citations in OpenAlex.
- A complex between IF2 and NusA suggests early coupling of transcription-translation.Nature communications · 2025Article
- Assembly and analysis of the complete mitochondrial genome of an endemic Camellia species of China, Camellia tachangensis.BMC genomics · 2025Article
- Systematic analysis of tRNA transcription unit deletions in E. coli reveals insights into tRNA gene essentiality and cellular adaptation.Scientific reports · 2024Article
- Physiological significance of the two isoforms of initiator tRNAs inJournal of bacteriology · 2024Article
- MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin binding.Nature communications · 2023Article
- Lamotrigine compromises the fidelity of initiator tRNA recruitment to the ribosomal P-site by IF2 and the RbfA release from 30S ribosomes inRNA biology · 2023Article
- RNA modifications stabilize the tertiary structure of tRNAfMet by locally increasing conformational dynamics.Nucleic acids research · 2022Article
- Identification and characterization of VapBC toxin-antitoxin system inRNA (New York, N.Y.) · 2021Article
- Initiating protein synthesis with noncanonical monomers in vitro and in vivo.Methods in enzymology · 2021Article
- Transcriptional and post-transcriptional events trigger de novo infB expression in cold stressed Escherichia coli.Nucleic acids research · 2019Article
- Rapid formylation of the cellular initiator tRNA population makes a crucial contribution to its exclusive participation at the step of initiation.Nucleic acids research · 2019Article
- Toxins targeting transfer RNAs: Translation inhibition by bacterial toxin-antitoxin systems.Wiley interdisciplinary reviews. RNA · 2019Review
- The structure of anRNA (New York, N.Y.) · 2017Article
- Two highly conserved features of bacterial initiator tRNAs license them to pass through distinct checkpoints in translation initiation.Nucleic acids research · 2017Article
- An evolutionarily conserved element in initiator tRNAs prompts ultimate steps in ribosome maturation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Evolution of initiator tRNAs and selection of methionine as the initiating amino acid.RNA biology · 2016Article
- Is the cellular initiation of translation an exclusive property of the initiator tRNAs?RNA biology · 2015Article
- An extended Shine-Dalgarno sequence in mRNA functionally bypasses a vital defect in initiator tRNA.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- How many initiator tRNA genes does Escherichia coli need?Journal of bacteriology · 2014Article
- Initiation with elongator tRNAs.Journal of bacteriology · 2013Article
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The accuracy of the initiator tRNA (tRNA(fMet)) selection in the ribosomal P-site is central to the fidelity of protein synthesis. A highly conserved occurrence of three consecutive G-C base pairs in the anticodon stem of tRNA(fMet) contributes to its preferential selection in the P-site. In a genetic screen, using a plasmid borne copy of an inactive tRNA(fMet) mutant wherein the three G-C base pairs were changed, we isolated Escherichia coli strains that allow efficient initiation with the tRNA(fMet) mutant. Here, extensive characterization of two such strains revealed novel mutations in the metZWV promoter severely compromising tRNA(fMet) levels. Low cellular abundance of the chromosomally encoded tRNA(fMet) allows efficient initiation with the tRNA(fMet) mutant and an elongator tRNA(Gln), revealing that a high abundance of the cellular tRNA(fMet) is crucial for the fidelity of initiator tRNA selection on the ribosomal P-site in E. coli. We discuss possible implications of the changes in the cellular tRNA(fMet) abundance in proteome remodeling.
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