ArticleCurrent biology : CB2010
Mitotic kinesin CENP-E promotes microtubule plus-end elongation.
Article in Current biology : CB, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 65 citations in OpenAlex.
- Kinesin-7 CENP-E mediates chromosome alignment and spindle assembly checkpoint in meiosis I.Chromosoma · 2024Article
- The motor domain of the kinesin Kip2 promotes microtubule polymerization at microtubule tips.The Journal of cell biology · 2023Article
- Determinant factors for residence time of kinesin motors at microtubule ends.Journal of biological physics · 2023Article
- Reconstitution of an active human CENP-E motor.Open biology · 2022Article
- Inhibiting microcephaly genes as alternative to microtubule targeting agents to treat brain tumors.Cell death & disease · 2021Review
- CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells.Cancers · 2021Article
- Leaving no-one behind: how CENP-E facilitates chromosome alignment.Essays in biochemistry · 2020Review
- CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis.World journal of surgical oncology · 2020Article
- Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells.Cell death discovery · 2020Article
- Complex Interplay Among Nuclear Receptor Ligands, Cytosine Methylation, and the Metabolome in Driving Tris(1,3-dichloro-2-propyl)phosphate-Induced Epiboly Defects in Zebrafish.Environmental science & technology · 2019Article
- Identification of key residues that regulate the interaction of kinesins with microtubule ends.Cytoskeleton (Hoboken, N.J.) · 2019Article
- GSK923295 as a potential antihepatocellular carcinoma agent causing delay on liver regeneration after partial hepatectomy.Chinese medical journal · 2019Article
- Microtubule dynamics: an interplay of biochemistry and mechanics.Nature reviews. Molecular cell biology · 2018Review
- Altered chemomechanical coupling causes impaired motility of the kinesin-4 motors KIF27 and KIF7.The Journal of cell biology · 2018Article
- A centrosomal protein STARD9 promotes microtubule stability and regulates spindle microtubule dynamics.Cell cycle (Georgetown, Tex.) · 2018Article
- Common general anesthetic propofol impairs kinesin processivity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.Cell death & disease · 2016Article
- The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition.Open biology · 2016Article
- Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.The Journal of biological chemistry · 2016Article
- Kinesin-5 is a microtubule polymerase.Nature communications · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Centromere protein CENP-E is a dimeric kinesin (Kinesin-7 family) with critical roles in mitosis, including establishment of microtubule (MT)-chromosome linkage and movement of mono-oriented chromosomes on kinetochore microtubules for proper alignment at metaphase [1-9]. We performed studies to test the hypothesis that CENP-E promotes MT elongation at the MT plus ends. A human CENP-E construct was engineered, expressed, and purified, and it yielded the CENP-E-6His dimeric motor protein. The results show that CENP-E promotes MT plus-end-directed MT gliding at 11 nm/s. The results from real-time microscopy assays indicate that 60.3% of polarity-marked MTs exhibited CENP-E-promoted MT plus-end elongation. The MT extension required ATP turnover, and MT plus-end elongation occurred at 1.48 μm/30 min. Immunolocalization studies revealed that 80.8% of plus-end-elongated MTs showed CENP-E at the MT plus end. The time dependence of CENP-E-promoted MT elongation in solution best fit a single exponential function (k(obs) = 5.1 s(-1)), which is indicative of a mechanism in which α,β-tubulin subunit addition is tightly coupled to ATP turnover. Based on these results, we propose that CENP-E, as part of its function in chromosome kinetochore-MT linkage, plays a direct role in MT elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.