ArticleCurrent eye research2010
Assessment of Streptococcus pneumoniae capsule in conjunctivitis and keratitis in vivo neuraminidase activity increases in nonencapsulated pneumococci following conjunctival infection.
Article in Current eye research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- High-resolution genomics uncovers region-specific evolution and virulence of ocularMicrobiology spectrum · 2026Article
- Prevalence of adenoviruses as ocular disease causatives in Saudi Arabia.Saudi journal of biological sciences · 2022Review
- Absence ofMicroorganisms · 2022Article
- Foundational concepts in the biology of bacterial keratitis.Experimental eye research · 2021Review
- The Rcs Stress Response System Regulator GumB Modulates Serratia marcescens-Induced Inflammation and Bacterial Proliferation in a Rabbit Keratitis Model and CytotoxicityInfection and immunity · 2021Article
- Predominant role of Haemophilus influenzae in the association of conjunctivitis, acute otitis media and acute bacterial paranasal sinusitis in children.Scientific reports · 2021Article
- The Best of All Worlds:Microorganisms · 2019Review
- A Pyrrhic Victory: The PMN Response to Ocular Bacterial Infections.Microorganisms · 2019Review
- The Role of Pneumococcal Virulence Factors in Ocular Infectious Diseases.Interdisciplinary perspectives on infectious diseases · 2018Review
- Puzzling Over the Pneumococcal Pangenome.Frontiers in microbiology · 2018Review
- Molecular characterization of pneumococcal surface protein K, a potential pneumococcal vaccine antigen.Virulence · 2017Article
- Review
- Article
- Proteomics in the Study of Bacterial Keratitis.Proteomes · 2015Review
- Infectious keratitis: secreted bacterial proteins that mediate corneal damage.Journal of ophthalmology · 2013Article
- Active Immunization with Pneumolysin versus 23-Valent Polysaccharide Vaccine for Streptococcus pneumoniae Keratitis.Investigative ophthalmology & visual science · 2011Article
- The Streptococcus pneumoniae capsule is required for full virulence in pneumococcal endophthalmitis.Investigative ophthalmology & visual science · 2011Article
- Pathogenesis of A Clinical Ocular Strain of Streptococcus pneumoniae and the Interaction of Pneumolysin with Corneal Cells.Journal of bacteriology & parasitology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe pneumococcal capsule is required for pathogenesis in systemic infections, yet reports show most conjunctivitis outbreaks are caused by nonencapsulated pneumococci, while keratitis infections are caused by encapsulated strains. This study aims to determine the effect of capsule in pneumococcal keratitis and conjunctivitis in rabbit models of infection.
methodsA capsule-deficient isogenic mutant was created using homologous transformation. Parent and mutant strains were injected within the upper bulbar conjunctiva (conjunctivitis) or into the corneal stroma (keratitis) of New Zealand white rabbits. Clinical examinations were performed 24 and 48 hr post-infection at which time corneas or conjunctivae were removed, homogenized, and plated to determine the recovered bacterial load. Whole eyes were removed for histological examination. The neuraminidase activity was determined following in vitro and in vivo growth.
resultsThere were no significant differences in clinical scores between the eyes infected with the parent or mutant for either infection, nor was there a difference in the amount of bacteria recovered from the cornea. In the conjunctivae, however, the mutant strain was cleared by the host faster than the parent strain. Histological examination showed slightly more infiltrating polymorphonuclear leukocytes (PMN) and macrophages in the conjunctivae infected with the parent strain. The neuraminidase activity of both strains was not significantly different when the strains were grown in vitro. However, the neuraminidase activity of the parent was significantly less than that of the mutant at 3 and 12 hr post conjunctival infection.
conclusionsAlthough more outbreaks of pneumococcal conjunctivitis are tied to nonencapsulated S. pneumoniae strains, this study showed that an encapsulated strain was capable of establishing conjunctivitis in a rabbit injection model and survive attack by the host immune system longer than its nonencapsulated isogenic mutant. Nonetheless, the nonencapsulated pneumococci had an increased neuraminidase activity level in vivo when compared to the parent strain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.