ArticleBiochemistry2010
Escherichia coli mutants that synthesize dephosphorylated lipid A molecules.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Dark Side ofJournal of the American Chemical Society · 2026Article
- Delivery ofmSphere · 2024Article
- Induction of Protective Antiplague Immune Responses by Self-Adjuvanting Bionanoparticles Derived from Engineered Yersinia pestis.Infection and immunity · 2020Article
- Phylogenetic barriers to horizontal transfer of antimicrobial peptide resistance genes in the human gut microbiota.Nature microbiology · 2019Article
- Proof of an Outer Membrane Target of the Efflux Inhibitor Phe-Arg-β-Naphthylamide from Random Mutagenesis.Molecules (Basel, Switzerland) · 2019Article
- Biosynthesis and structure-activity relationships of the lipid a family of glycolipids.Current opinion in chemical biology · 2017Review
- Article
- Modification of the 1-Phosphate Group during Biosynthesis of Capnocytophaga canimorsus Lipid A.Infection and immunity · 2016Article
- Mechanisms of Antimicrobial Peptide Resistance in Gram-Negative Bacteria.Antibiotics (Basel, Switzerland) · 2015Article
- Pathogenicity of Yersinia pestis synthesis of 1-dephosphorylated lipid A.Infection and immunity · 2013Article
- Modulating the innate immune response by combinatorial engineering of endotoxin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Phosphate groups of lipid A are essential for Salmonella enterica serovar Typhimurium virulence and affect innate and adaptive immunity.Infection and immunity · 2012Article
- The calcium-stimulated lipid A 3-O deacylase from Rhizobium etli is not essential for plant nodulation.Biochimica et biophysica acta · 2012Article
- Helicobacter pylori versus the host: remodeling of the bacterial outer membrane is required for survival in the gastric mucosa.PLoS pathogens · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The lipid A moiety of Escherichia coli lipopolysaccharide is a hexaacylated disaccharide of glucosamine that is phosphorylated at the 1 and 4' positions. Expression of the Francisella novicida lipid A 1-phosphatase FnLpxE in E. coli results in dephosphorylation of the lipid A proximal unit. Coexpression of FnLpxE and the Rhizobium leguminosarum lipid A oxidase RlLpxQ in E. coli converts much of the proximal glucosamine to 2-amino-2-deoxygluconate. Expression of the F. novicida lipid A 4'-phosphatase FnLpxF in wild-type E. coli has no effect because FnLpxF cannot dephosphorylate hexaacylated lipid A. However, expression of FnLpxF in E. coli lpxM mutants, which synthesize pentaacylated lipid A lacking the secondary 3'-myristate chain, causes extensive 4'-dephosphorylation. Coexpression of FnLpxE and FnLpxF in lpxM mutants results in massive accumulation of lipid A species lacking both phosphate groups, and introduction of RlLpxQ generates phosphate-free lipid A variants containing 2-amino-2-deoxygluconate. The proposed lipid A structures were confirmed by electrospray ionization mass spectrometry. Strains with 4'-dephosphorylated lipid A display increased polymyxin resistance. Heptose-deficient mutants of E. coli lacking both the 1- and 4'-phosphate moieties are viable on plates but sensitive to CaCl(2). Our methods for reengineering lipid A structure may be useful for generating novel vaccines and adjuvants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.