ArticleJournal of molecular neuroscience : MN1990
Platelet-activating factor activates HIV promoter in transfected SH-SY5Y neuroblastoma cells and MOLT-4 T lymphocytes.
Article in Journal of molecular neuroscience : MN, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Lipid signaling in neural plasticity, brain repair, and neuroprotection.Molecular neurobiology · 2005Review
- Expression of human platelet-activating factor receptor gene in EoL-1 cells following butyrate-induced differentiation.The Biochemical journal · 1995Article
- Platelet-activating factor and retinoic acid synergistically activate the inducible prostaglandin synthase gene.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Platelet-activating factor induces collagenase expression in corneal epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- A platelet-activating factor antagonist, RP 55778, inhibits cytokine-dependent induction of human immunodeficiency virus expression in chronically infected promonocytic cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Excitable membranes, lipid messengers, and immediate-early genes. Alteration of signal transduction in neuromodulation and neurotrauma.Molecular neurobiology · 1992Review
- Review
- Review
- Fos-jun and the primary genomic response in the nervous system. Possible physiological role and pathophysiological significance.Molecular neurobiologyReview
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transfected gene constructs comprising the long terminal repeat (LTR) sequence of the human immunodeficiency virus (HIV) genome spliced to an assayable reporter gene have made possible the evaluation of a lipid mediator, platelet-activating factor (PAF), as a potential HIV transcriptional regulatory molecule. We assessed the activation of the HIV LTR promoter sequence linked to the chloramphenicol acetyltransferase (CAT) reporter gene (HIV-CAT) by PAF in both a human neural (SH-SY5Y neuroblastoma) and a human leukocytic (MOLT-4 T-lymphocyte) cell line. PAF activated expression of the HIV-CAT construct in both the SH-SY5Y and MOLT-4 T-cell lines. PAF-induced CAT activity was approximately six to seven times higher in the SH-SY5Y cells than in the MOLT-4 cells. Preincubation of cells with the specific PAF antagonist BN 52021 completely inhibited CAT expression in both cell lines. The biologically inactive PAF precursor lyso-PAF did not activate CAT expression. Assays for CAT mRNA demonstrated an increase after PAF treatment, an effect that was completely inhibited by BN 52021, and which was not elicited by lyso-PAF. These results show that PAF represents a potential cellular mediator evoking the expression of the HIV genome.
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