ArticleGenetics2010
Functional dissection of IME1 transcription using quantitative promoter-reporter screening.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Destabilization of mRNAs enhances competence to initiate meiosis in mouse spermatogenic cells.Development (Cambridge, England) · 2024Article
- Gene regulation during meiosis.Trends in genetics : TIG · 2024Review
- Rie1 and Sgn1 form an RNA-binding complex that enforces the meiotic entry cell fate decision.The Journal of cell biology · 2023Article
- Modaline sulfate promotes Oct4 expression and maintains self-renewal and pluripotency of stem cells through JAK/STAT3 and Wnt signaling pathways.Cell & bioscience · 2021Article
- Transcription levels of a noncoding RNA orchestrate opposing regulatory and cell fate outcomes in yeast.Cell reports · 2021Article
- Regulated repression governs the cell fate promoter controlling yeast meiosis.Nature communications · 2020Article
- mNature communications · 2019Article
- Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway.PLoS genetics · 2017Article
- ER fatalities-The role of ER-mitochondrial contact sites in yeast life and death decisions.Mechanisms of ageing and development · 2017Review
- Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability.PLoS genetics · 2016Article
- Rpl22 is required for IME1 mRNA translation and meiotic induction in S. cerevisiae.Cell division · 2016Article
- The Rim15-endosulfine-PP2ACdc55 signalling module regulates entry into gametogenesis and quiescence via distinct mechanisms in budding yeast.PLoS genetics · 2014Article
- Mapping the fine structure of a eukaryotic promoter input-output function.Nature genetics · 2013Article
- The roles of the catalytic and noncatalytic activities of Rpd3L and Rpd3S in the regulation of gene transcription in yeast.PloS one · 2013Article
- Multiple MAPK cascades regulate the transcription of IME1, the master transcriptional activator of meiosis in Saccharomyces cerevisiae.PloS one · 2013Article
- Erroneous attribution of relevant transcription factor binding sites despite successful prediction of cis-regulatory modules.BMC genomics · 2011Article
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional regulation is a key mechanism that controls the fate and response of cells to diverse signals. Therefore, the identification of the DNA-binding proteins, which mediate these signals, is a crucial step in elucidating how cell fate is regulated. In this report, we applied both bioinformatics and functional genomic approaches to scrutinize the unusually large promoter of the IME1 gene in budding yeast. Using a recently described fluorescent protein-based reporter screen, reporter-synthetic genetic array (R-SGA), we assessed the effect of viable deletion mutants on transcription of various IME1 promoter-reporter genes. We discovered potential transcription factors, many of which have no perfect consensus site within the IME1 promoter. Moreover, most of the cis-regulatory sequences with perfect homology to known transcription factor (TF) consensus were found to be nonfunctional in the R-SGA analysis. In addition, our results suggest that lack of conservation may not discriminate against a TF regulatory role at a specific promoter. We demonstrate that Sum1 and Sok2, which regulate IME1, bind to nonperfect consensuses within nonconserved regions in the sensu stricto Saccharomyces strains. Our analysis supports the view that although comparative analysis can provide a useful guide, functional assays are required for accurate identification of TF-binding site interactions in complex promoters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.