ArticleAmerican journal of physiology. Cell physiology2010
Dietary methionine restriction improves colon tight junction barrier function and alters claudin expression pattern.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 45 citations in OpenAlex.
- Dietary Methionine Restriction Improves Gut Health and Alters the Plasma Metabolomic Profile in Rats by Modulating the Composition of the Gut Microbiota.International journal of molecular sciences · 2024Article
- Dietary methionine sources and levels modulate the intestinal health status of broiler chickens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Effects on the Cell Barrier Function of L-Met and DL-HMTBA Is Related to Metabolic Characteristics and mFrontiers in nutrition · 2022Article
- Mitochondria-Microbiota Interaction in Neurodegeneration.Frontiers in aging neuroscience · 2021Review
- Perspective: Methionine Restriction-Induced Longevity-A Possible Role for Inhibiting the Synthesis of Bacterial Quorum Sensing Molecules.Advances in nutrition (Bethesda, Md.) · 2020Article
- Mouse Models of Human Claudin-Associated Disorders: Benefits and Limitations.International journal of molecular sciences · 2019Review
- Development of Salmonellosis as Affected by Bioactive Food Compounds.Microorganisms · 2019Review
- Article
- Review
- One-carbon metabolism and ionizing radiation: a multifaceted interaction.Biomolecular concepts · 2017Review
- Methionine restriction alters bone morphology and affects osteoblast differentiation.Bone reports · 2016Article
- Methionine restriction fundamentally supports health by tightening epithelial barriers.Annals of the New York Academy of Sciences · 2016Review
- Stochastic effects in adaptive reconstruction of body damage: implied the creativity of natural selection.Journal of cellular and molecular medicine · 2015Review
- Cellular polarity in aging: role of redox regulation and nutrition.Genes & nutrition · 2014Article
- Caloric restriction reduces edema and prolongs survival in a mouse glioma model.Journal of neuro-oncology · 2013Article
- Claudins in intestines: Distribution and functional significance in health and diseases.Tissue barriers · 2013Review
- Bin1 attenuation suppresses experimental colitis by enforcing intestinal barrier function.Digestive diseases and sciences · 2012Article
- Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart.Journal of bioenergetics and biomembranes · 2011Article
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8 authors at 2 institutions in 1 country.
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Abstract
The beneficial effects of caloric restriction in increasing longevity and forestalling age-related diseases are well known. Dietary restriction of methionine also renders similar benefits. We recently showed in a renal epithelial cell culture system that reduction of culture medium methionine by 80% resulted in altered tight junctional (TJ) claudin composition and also improved epithelial barrier function (51). In the current study, we examined the effect of dietary restriction of methionine on TJ barrier function in rat gastrointestinal tissue to see whether this phenomenon also holds true in a tissue model and for a different epithelial cell type. After 28 days on methionine-restricted (MR) diet, rats showed small but significant reductions in the plasma and (intracellular) colonocyte levels of methionine. Colon mucosal sheets from rats on the MR diet showed increased transepithelial electrical resistance with concomitant decrease in paracellular diffusion of (14)C-D-mannitol, suggesting improved barrier function relative to rats on control diet. This improved barrier function could not be explained by changes in colon crypt length or frequency. Neither was the colonocyte mitotic index nor the apoptotic frequency altered significantly. However, TJ composition/structure was being altered by the MR diet. RT-PCR and Western blot analysis showed an increase in the abundance of claudin-3 and an apparent change in the posttranslational modification of occludin, data reinforcing a paracellular barrier alteration. Overall, our data suggest that reduction in dietary intake of methionine results in improved epithelial barrier function by inducing altered TJ protein composition.
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