ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2010
Cloning and characterization of a zebrafish homologue of human AQP1: a bifunctional water and gas channel.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 43 citations in OpenAlex.
- Expression of genes encoding non-specific immunity, anti-oxidative status and aquaporins in β-glucan-fed golden mahseer (Fish and shellfish immunology reports · 2023Article
- Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2022Article
- Aquaporin expression and cholesterol content in eel swimbladder tissue.Journal of fish biology · 2022Article
- CO2 induced seawater acidification impacts survival and development of European eel embryos.PloS one · 2022Article
- The digestive tract as an essential organ for water acquisition in marine teleosts: lessons from euryhaline eels.Zoological letters · 2021Review
- Differential Expression and Localization of Branchial AQP1 and AQP3 in Japanese Medaka (Cells · 2019Article
- Plant and Mammal Aquaporins: Same but Different.International journal of molecular sciences · 2018Review
- Molecular Characterization of Aquaporin 1 and Aquaporin 3 from the Gills of the African Lungfish,Frontiers in physiology · 2016Article
- Mathematical modeling of acid-base physiology.Progress in biophysics and molecular biology · 2015Review
- Aquaporin 1 Is Involved in Acid Secretion by Ionocytes of Zebrafish Embryos through Facilitating CO2 Transport.PloS one · 2015Article
- Evidence from simultaneous intracellular- and surface-pH transients that carbonic anhydrase II enhances CO2 fluxes across Xenopus oocyte plasma membranes.American journal of physiology. Cell physiology · 2014Article
- The role of aquaporin and tight junction proteins in the regulation of water movement in larval zebrafish (Danio rerio).PloS one · 2013Article
- Role of an extracellular loop in determining the stoichiometry of Na+-HCO₃⁻ cotransporters.The Journal of physiology · 2011Article
- Role of Aquaporins during Teleost Gametogenesis and Early Embryogenesis.Frontiers in physiology · 2011Article
- Sharpey-Schafer lecture: gas channels.Experimental physiology · 2010Article
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
The mammalian aquaporins AQP1, AQP4, and AQP5 have been shown to function not only as water channels but also as gas channels. Zebrafish have two genes encoding an AQP1 homologue, aqp1a and aqp1b. In the present study, we cloned the cDNA that encodes the zebrafish protein Aqp1a from the 72-h postfertilization (hpf) embryo of Danio rerio, as well as from the swim bladder of the adult. The deduced amino-acid sequence of aqp1a consists of 260 amino acids and is 59% identical to human AQP1. By analyzing the genomic DNA sequence, we identified four exons in the aqp1a gene. By in situ hybridization, aqp1a is expressed transiently in the developing vasculature and in erythrocytes from 16 to 48 h of development. Later, at 72 hpf, aqp1a is expressed in dermal ionocytes and in the swim bladder. Western blot analysis of adult tissues reveals that Aqp1a is most highly expressed in the eye and swim bladder. Xenopus oocytes expressing aqp1a have a channel-dependent (*) osmotic water permeability (P(f)(*)) that is indistinguishable from that of human AQP1. On the basis of the magnitude of the transient change in surface pH (ΔpH(S)) that were recorded as the oocytes were exposed to either CO(2) or NH(3), we conclude that zebrafish Aqp1a is permeable to both CO(2) and NH(3). The ratio (ΔpH(S)(*))((CO)2)/P(f)(*) is about half that of human AQP1, and the ratio (ΔpH(S)(*))(NH3)/P(f)(*) is about one-quarter that of human AQP1. Thus, compared with human AQP1, zebrafish Aqp1a has about twice the selectivity for CO(2) over NH(3).
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