Evidence map›Paper›PMID 20739533›Full record

ArticleJournal of virology2010

Compromised spindle assembly checkpoint due to altered expression of Ubch10 and Cdc20 in human papillomavirus type 16 E6- and E7-expressing keratinocytes.

Daksha Patel, Dennis J McCance

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Review
  3. Stabilization of p27FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019
    Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Ubiquitin-conjugating enzyme UBE2C: molecular biology, role in tumorigenesis, and potential as a biomarker.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012
    Review
  12. Immunohistochemical analysis of the ubiquitin-conjugating enzyme UbcH10 in lung cancer: a useful tool for diagnosis and therapy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012
    Article
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Daksha PatelCentre for Cancer Research and Cell Biology, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom. d.patel@qub.ac.uk
Dennis J McCance
Queen's University Belfast · GB

Funding

Study of genomic instability caused by HPV16 E6 and E7R01DE015935 · NIDCR · UNIVERSITY OF ROCHESTER · PI MCCANCE, DENNIS J. · 2004 to 2008
$1.6M
Mitotic Spindle Checkpoint Regulation by HPV E6 and E7R03DE015452 · NIDCR · UNIVERSITY OF ROCHESTER · PI PATEL, DAKSHA · 2003 to 2004
$157k
NIDCR NIH HHS 5R01DE015935-05NIDCR NIH HHS 5R03 DE015452-02NIDCR NIH HHS R01 DE015935NIDCR NIH HHS R03 DE015452
6 · The paper itself

Abstract

Cells expressing human papillomavirus type 16 (HPV-16) E6 and E7 proteins exhibit deregulation of G2/M genes, allowing bypass of DNA damage arrest signals. Normally, cells with DNA damage that override the G2 damage checkpoint would precociously enter mitosis and ultimately face mitotic catastrophe and apoptotic cell death. However, E6/E7-expressing cells (E6/E7 cells) have the ability to enter and exit mitosis in the presence of DNA damage and continue with the next round of the cell cycle. Little is known about the mechanism that allows these cells to gain entry into and exit from mitosis. Here, we show that in the presence of DNA damage, E6/E7 cells have elevated levels of cyclin B, which would allow entry into mitosis. Also, as required for exit from mitosis, cyclin B is degraded in these cells, permitting initiation of the next round of DNA synthesis and cell cycle progression. Proteasomal degradation of cyclin B by anaphase-promoting complex/cyclosome (APC/C) is, in part, due to elevated levels of the E2-conjugating enzyme, Ubch10, and the substrate recognition protein, Cdc20, of APC/C. Also, in E6/E7 cells with DNA damage, while Cdc20 is complexed with BubR1, indicating an active checkpoint, it is also present in complexes free of BubR1, presumably allowing APC/C activity and slippage through the checkpoint.

Indexed as

Gene Expression RegulationCdc20 ProteinsCell CycleCell Cycle ProteinsCyclin BDNA DamageHuman papillomavirus 16HumansKeratinocytesMitosisOncogene Proteins, ViralPapillomavirus E7 ProteinsRepressor ProteinsSpindle ApparatusUbiquitin-Conjugating EnzymesCDC20 protein, humanCdc20 ProteinsCell Cycle ProteinsCyclin BE6 protein, Human papillomavirus type 16oncogene protein E7, Human papillomavirus type 16Oncogene Proteins, ViralPapillomavirus E7 ProteinsRepressor ProteinsUBE2C protein, humanUbiquitin-Conjugating Enzymes

Identifiers

PMID20739533
PMCPMC2953194
OpenAlexW2122439998

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.