ArticleJournal of clinical microbiology2010
Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Chromogenic hydroxyanthraquinone-based enzyme substrates for the detection of microbial β-d-galactosidase, β-d-glucuronidase and β-d-ribosidase.RSC advances · 2025Article
- Emerging Diagnostics inInternational journal of molecular sciences · 2024Review
- The Brief Case: a White-Colony-Producing Clostridioides difficile Ribotype 020 Strain.Journal of clinical microbiology · 2023Review
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridioides) difficile from the environment in Western Australia.Journal of applied microbiology · 2022Article
- Efficacy of Dry Heat Treatment againstPathogens (Basel, Switzerland) · 2022Article
- Mild or Malign: Clinical Characteristics and Outcomes of Clostridium difficile Infection in Thailand.Journal of clinical microbiology · 2020Article
- A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics.Clinical microbiology reviews · 2017Review
- Current knowledge on the laboratory diagnosis ofWorld journal of gastroenterology · 2017Review
- Comparison of enzyme immunoassays and rapid diagnostic tests for clostridium difficile glutamate dehydrogenase and toxin a + B to toxinogenic culture on a highly selective chromogenic medium.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Isolation and Identification of Clostridium difficile Using ChromID C. difficile Medium Combined With Gram Staining and PRO Disc Testing: A Proposal for a Simple Culture Process.Annals of laboratory medicine · 2015Article
- Diagnosis of Clostridium difficile: real-time PCR detection of toxin genes in faecal samples is more sensitive compared to toxigenic culture.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Performance of chromID Clostridium difficile agar compared with BBL C. difficile selective agar for detection of C. difficile in stool specimens.Annals of laboratory medicine · 2014Article
- Evaluation of a chromogenic culture medium for the detection of Clostridium difficile.Yonsei medical journal · 2014Article
- A cost-effective approach for detection of toxigenic Clostridium difficile: toxigenic culture using ChromID Clostridium difficile agar.Journal of clinical microbiology · 2014Article
- Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.Annals of laboratory medicine · 2014Article
- Diagnosis of Clostridium difficile infection: an ongoing conundrum for clinicians and for clinical laboratories.Clinical microbiology reviews · 2013Review
- Evaluation of the fully automated BD MAX Cdiff and Xpert C. difficile assays for direct detection of Clostridium difficile in stool specimens.Journal of clinical microbiology · 2013Article
- Evaluation of the chromogenic agar chromID C. difficile.Journal of clinical microbiology · 2013Article
- Use of culture- and ELISA-based toxin assay for detectingJournal of laboratory physiciansArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and effective methods for the isolation of Clostridium difficile from stool samples are desirable to obtain isolates for typing or to facilitate accurate diagnosis of C. difficile-associated diarrhea. We report on the evaluation of a prototype chromogenic medium (ID C. difficile prototype [IDCd]) for isolation of C. difficile. The chromogenic medium was compared using (i) 368 untreated stool samples that were also inoculated onto CLO medium, (ii) 339 stool samples that were subjected to alcohol shock and also inoculated onto five distinct selective agars, and (iii) standardized suspensions of 10 C. difficile ribotypes (untreated and alcohol treated) that were also inoculated onto five distinct selective agars. Two hundred thirty-six isolates of C. difficile were recovered from 368 untreated stool samples, and all but 1 of these strains (99.6%) were recovered on IDCd within 24 h, whereas 74.6% of isolates were recovered on CLO medium after 48 h. Of 339 alcohol-treated stool samples cultured onto IDCd and five other selective agars, C. difficile was recovered from 218 samples using a combination of all media. The use of IDCd allowed recovery of 96.3% of isolates within 24 h, whereas 51 to 83% of isolates were recovered within 24 h using the five other media. Finally, when they were challenged with pure cultures, all 10 ribotypes of C. difficile generated higher colony counts on IDCd irrespective of alcohol pretreatment or duration of incubation. We conclude that IDCd is an effective medium for isolation of C. difficile from stool samples within 24 h.
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