ArticleThe Journal of physiology2010
Transgene expression and effective gene silencing in vagal afferent neurons in vivo using recombinant adeno-associated virus vectors.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- Gut-brain communication: types of sensory nerves and mechanisms of activation.Nature reviews. Gastroenterology & hepatology · 2026Review
- Capsaicin-Sensitive Vagal Afferent Nerve-Mediated Interoceptive Signals in the Esophagus.Molecules (Basel, Switzerland) · 2021Review
- Vagus Nerves Provide a Robust Afferent Innervation of the Mucosa Throughout the Body of the Esophagus in the Mouse.Dysphagia · 2020Article
- Dissecting the Role of Subtypes of Gastrointestinal Vagal Afferents.Frontiers in physiology · 2020Review
- Molecular/Ionic Basis of Vagal Bronchopulmonary C-Fiber Activation by Inflammatory Mediators.Physiology (Bethesda, Md.) · 2020Review
- Avoiding off-target effects in electrical stimulation of the cervical vagus nerve: Neuroanatomical tracing techniques to study fascicular anatomy of the vagus nerve.Journal of neuroscience methods · 2019Review
- Structure of vagal afferent nerve terminal fibers in the mouse trachea.Respiratory physiology & neurobiology · 2018Article
- Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells.Molecular therapy. Methods & clinical development · 2017Article
- Generation and validation of novel adeno-associated viral vectors for the analysis of CaScientific reports · 2017Article
- Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors.Current opinion in biotechnology · 2016Review
- Vagal Afferent Innervation of the Airways in Health and Disease.Physiological reviews · 2016Review
- TRP channel functions in the gastrointestinal tract.Seminars in immunopathology · 2016Review
- Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping.Nature protocols · 2015Article
- Peripheral neural circuitry in cough.Current opinion in pharmacology · 2015Review
- Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression.PloS one · 2015Article
- A method of nodose ganglia injection in Sprague-Dawley rat.Journal of visualized experiments : JoVE · 2014Article
- Biodistribution of adeno-associated virus serotype 9 (AAV9) vector after intrathecal and intravenous delivery in mouse.Frontiers in neuroanatomy · 2014Article
- Selective inhibition of vagal afferent nerve pathways regulating cough using Nav 1.7 shRNA silencing in guinea pig nodose ganglia.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Visualization of spinal afferent innervation in the mouse colon by AAV8-mediated GFP expression.Neurogastroenterology and motility · 2013Article
- Molecular anatomy of the gut-brain axis revealed with transgenic technologies: implications in metabolic research.Frontiers in neuroscience · 2013Article
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9 authors at 3 institutions in 2 countries.
Funding
Abstract
Vagal afferent fibres innervating thoracic structures such as the respiratory tract and oesophagus are diverse, comprising several subtypes of functionally distinct C-fibres and A-fibres. Both morphological and functional studies of these nerve subtypes would be advanced by selective, effective and long-term transduction of vagal afferent neurons with viral vectors. Here we addressed the hypothesis that vagal sensory neurons can be transduced with adeno-associated virus (AAV) vectors in vivo, in a manner that would be useful for morphological assessment of nerve terminals, using enhanced green fluorescent protein (eGFP), as well as for the selective knock-down of specific genes of interest in a tissue-selective manner. We found that a direct microinjection of AAV vectors into the vagal nodose ganglia in vivo leads to selective, effective and long-lasting transduction of the vast majority of primary sensory vagal neurons without transduction of parasympathetic efferent neurons. The transduction of vagal neurons by pseudoserotype AAV2/8 vectors in vivo is sufficiently efficient such that it can be used to functionally silence TRPV1 gene expression using short hairpin RNA (shRNA). The eGFP encoded by AAV vectors is robustly transported to both the central and peripheral terminals of transduced vagal afferent neurons allowing for bright imaging of the nerve endings in living tissues and suitable for structure-function studies of vagal afferent nerve endings. Finally, the AAV2/8 vectors are efficiently taken up by the vagal nerve terminals in the visceral tissue and retrogradely transported to the cell body, allowing for tissue-specific transduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.