ArticleRNA (New York, N.Y.)2010
Differential assembly of 16S rRNA domains during 30S subunit formation.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Structural adaptations for enhanced translation kinetics in evolved ribosomes.Nucleic acids research · 2026Article
- Structural adaptations for enhanced translation kinetics in evolved ribosomes.bioRxiv : the preprint server for biology · 2026Article
- Exploring molecular mechanisms of aminoglycoside resistance in Escherichia coli MG1655 using the antibiotic resistance growth plate.Scientific reports · 2026Article
- Single Mutation inMicroorganisms · 2025Article
- Variable Region Sequences Influence 16S rRNA Performance.Microbiology spectrum · 2023Article
- Ψ-Footprinting approach for the identification of protein synthesis inhibitor producers.NAR genomics and bioinformatics · 2022Article
- Hibernation factors directly block ribonucleases from entering the ribosome in response to starvation.Nucleic acids research · 2021Article
- Network theory of the bacterial ribosome.PloS one · 2020Article
- Molecular Phylogenetic Analysis ofInternational journal of microbiology · 2020Article
- Dissecting macromolecular recognition sites in ribosome: implication to its self-assembly.RNA biology · 2019Article
- Temperature-dependent expression of different guanine-plus-cytosine content 16S rRNA genes in Haloarcula strains of the class Halobacteria.Antonie van Leeuwenhoek · 2019Article
- A metastable rRNA junction essential for bacterial 30S biogenesis.Nucleic acids research · 2018Article
- Comparative RNA function analysis reveals high functional similarity between distantly related bacterial 16 S rRNAs.Scientific reports · 2017Article
- RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket.Nucleic acids research · 2017Article
- Model-Free RNA Sequence and Structure Alignment Informed by SHAPE Probing Reveals a Conserved Alternate Secondary Structure for 16S rRNA.PLoS computational biology · 2015Article
- Multiple in vivo pathways for Escherichia coli small ribosomal subunit assembly occur on one pre-rRNA.Nature structural & molecular biology · 2014Article
- Ribosome RNA assembly intermediates visualized in living cells.Biochemistry · 2014Article
- RNA folding pathways and the self-assembly of ribosomes.Accounts of chemical research · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Rapid and accurate assembly of the ribosomal subunits, which are responsible for protein synthesis, is required to sustain cell growth. Our best understanding of the interaction of 30S ribosomal subunit components (16S ribosomal RNA [rRNA] and 20 ribosomal proteins [r-proteins]) comes from in vitro work using Escherichia coli ribosomal components. However, detailed information regarding the essential elements involved in the assembly of 30S subunits still remains elusive. Here, we defined a set of rRNA nucleotides that are critical for the assembly of the small ribosomal subunit in E. coli. Using an RNA modification interference approach, we identified 54 nucleotides in 16S rRNA whose modification prevents the formation of a functional small ribosomal subunit. The majority of these nucleotides are located in the head and interdomain junction of the 30S subunit, suggesting that these regions are critical for small subunit assembly. In vivo analysis of specific identified sites, using engineered mutations in 16S rRNA, revealed defective protein synthesis capability, aberrant polysome profiles, and abnormal 16S rRNA processing, indicating the importance of these residues in vivo. These studies reveal that specific segments of 16S rRNA are more critical for small subunit assembly than others, and suggest a hierarchy of importance.
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