ArticleMolecular microbiology2010
Purine restriction induces pronounced translational upregulation of the NT1 adenosine/pyrimidine nucleoside transporter in Leishmania major.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Nutrient sensing in Leishmania: Flagellum and cytosol.Molecular microbiology · 2021Review
- Deletion of a Single LeishIF4E-3 Allele by the CRISPR-Cas9 System Alters Cell Morphology and Infectivity ofmSphere · 2019Article
- Characterization of a Novel Endoplasmic Reticulum Protein Involved in Tubercidin Resistance in Leishmania major.PLoS neglected tropical diseases · 2016Article
- Regulation and biological function of a flagellar glucose transporter in Leishmania mexicana: a potential glucose sensor.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Identification of the intracellular gate for a member of the equilibrative nucleoside transporter (ENT) family.The Journal of biological chemistry · 2014Article
- Metabolic reprogramming during purine stress in the protozoan pathogen Leishmania donovani.PLoS pathogens · 2014Article
- Cysteine cross-linking defines the extracellular gate for the Leishmania donovani nucleoside transporter 1.1 (LdNT1.1).The Journal of biological chemistry · 2012Article
- Leishmania metacyclogenesis is promoted in the absence of purines.PLoS neglected tropical diseases · 2012Article
- Trypanocidal furamidine analogues: influence of pyridine nitrogens on trypanocidal activity, transport kinetics, and resistance patterns.Antimicrobial agents and chemotherapy · 2011Article
- Nutrient transport and pathogenesis in selected parasitic protozoa.Eukaryotic cell · 2011Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Leishmania and other parasitic protozoa are unable to synthesize purines de novo and are reliant upon purine nucleoside and nucleobase transporters to import preformed purines from their hosts. To study the roles of the four purine permeases NT1-NT4 in Leishmania major, null mutants in each transporter gene were prepared and the effect of each gene deletion on purine uptake was monitored. Deletion of the NT3 purine nucleobase transporter gene or both NT3 and the NT2 nucleoside transporter gene resulted in pronounced upregulation of adenosine and uridine uptake mediated by the NT1 permease and also induced up to a 200-fold enhancement in the level of the NT1 protein but not mRNA. A similar level of upregulation of NT1 was achieved in wild-type promastigotes that were transferred to medium deficient in purines. Pulse labelling and treatment of cells with the translation inhibitor cycloheximide revealed that control of NT1 expression occurs primarily at the level of translation and not protein turnover. These observations imply the existence of a translational control mechanism that enhances the ability of Leishmania parasites to import essential purines when they are present at limiting concentrations.
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