ArticleBritish journal of pharmacology2010
Adenosine and inosine release during hypoxia in the isolated spinal cord of neonatal rats.
Article in British journal of pharmacology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Short-Term Hypoxia Dampens Inflammation in vivo via Enhanced Adenosine Release and Adenosine 2B Receptor Stimulation.EBioMedicine · 2018Trial
- Differential expression, activity, and fibroblast growth factor 2-mediated modulation of purine-metabolizing enzymes in cultured cortical astrocytes and microglia.Purinergic signalling · 2026Article
- Persistent suppression of phrenic motor plasticity after mild acute systemic inflammation in adult rats.Function (Oxford, England) · 2026Article
- Caffeine and preterm infants: multiorgan effects and therapeutic creep: scope to optimise dose and timing.Pediatric research · 2026Review
- Acute intermittent hypoxia in neonatal rodent central nervous system facilitates respiratory frequency through the recruitment of hypothalamic areas.Experimental physiology · 2025Article
- Comparative analysis of adenosine 1 receptor expression and function in hippocampal and hypothalamic neurons.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025Article
- Caffeine and Its Neuroprotective Role in Ischemic Events: A Mechanism Dependent on Adenosine Receptors.Cellular and molecular neurobiology · 2022Review
- Thiamine as a Possible Neuroprotective Strategy in Neonatal Hypoxic-Ischemic Encephalopathy.Antioxidants (Basel, Switzerland) · 2021Review
- Adenosine A2a receptors modulate TrkB receptor-dependent respiratory plasticity in neonatal rats.Respiratory physiology & neurobiology · 2021Article
- Cervical spinal injury compromises caudal spinal tissue oxygenation and undermines acute intermittent hypoxia-induced phrenic long-term facilitation.Experimental neurology · 2021Article
- Urinary biomarkers indicative of recovery from spinal cord injury: A pilot study.IBRO neuroscience reports · 2021Article
- Novel Point-of-Care Diagnostic Method for Neonatal Encephalopathy Using Purine Nucleosides.Frontiers in molecular neuroscience · 2021Article
- Fibroblast growth factor 2 upregulates ecto-5'-nucleotidase and adenosine deaminase via MAPK pathways in cultured rat spinal cord astrocytes.Purinergic signalling · 2020Article
- Hypoxia-induced hypotension elicits adenosine-dependent phrenic long-term facilitation after carotid denervation.Experimental neurology · 2020Article
- Circulatory control of phrenic motor plasticity.Respiratory physiology & neurobiology · 2019Review
- One bout of neonatal inflammation impairs adult respiratory motor plasticity in male and female rats.eLife · 2019Article
- Activating adenosine A1 receptor accelerates PC12 cell injury via ADORA1/PKC/KATP pathway after intermittent hypoxia exposure.Molecular and cellular biochemistry · 2018Article
- Metabolic modeling helps interpret transcriptomic changes during malaria.Biochimica et biophysica acta. Molecular basis of disease · 2018Article
- Gliotransmission and adenosinergic modulation: insights from mammalian spinal motor networks.Journal of neurophysiology · 2017Review
- Profound hypothermia after adenosine kinase inhibition in A1AR-deficient mice suggests a receptor-independent effect of intracellular adenosine.Pflugers Archiv : European journal of physiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeAdenosine and inosine accumulate extracellularly during hypoxia/ischaemia in the brain and may act as neuroprotectants. In spinal cord, there is pharmacological evidence for increases in extracellular adenosine during hypoxia, but no direct measurements of purine release. Furthermore, the efflux pathways and origin of extracellular purines are not defined. To characterize hypoxia-evoked purine accumulation, we examined the effect of acute hypoxia on the extracellular levels of adenosine and inosine in isolated spinal cords from rats. EXPERIMENTAL APPROACH: Extracellular adenosine and inosine concentrations were assayed in an in vitro preparation of the isolated spinal cord of the neonatal rat by HPLC. KEY
resultsThe extracellular level of inosine was about 10-fold higher than that of adenosine. Acute hypoxia (10 min) caused a temperature-dependent increase in these two purines, which were inhibited by an increase in external Ca(2+), but not by several inhibitors of efflux pathways or metabolic enzymes of adenine nucleotides. Inhibitors of adenosine deaminase or the equilibrative nucleoside transporter (ENT) abolished the hypoxia-evoked increase in inosine but not adenosine. The inhibition of glial metabolism abolished the increase of both purines evoked by hypoxia but not by oxygen-glucose deprivation, hypercapnia or an adenosine kinase inhibitor. CONCLUSIONS AND IMPLICATIONS: Our data suggest that hypoxia releases adenosine itself from intracellular sources. Inosine formed intracellularly may be released through ENTs. During hypoxia, astrocytes appear to play a key role in purine release from neonatal rat spinal cord.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.