ArticleBiochemistry2010
Direct evidence of conformational heterogeneity in human pancreatic glucokinase from high-resolution nuclear magnetic resonance.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Characterizing glucokinase variant mechanisms using a multiplexed abundance assay.Genome biology · 2024Article
- A comprehensive map of human glucokinase variant activity.Genome biology · 2023Article
- Nuclear spin-hyperpolarization generated in a flavoprotein under illumination: experimental field-dependence and theoretical level crossing analysis.Scientific reports · 2019Article
- Tryptophan Fluorescence Yields and Lifetimes as a Probe of Conformational Changes in Human Glucokinase.Journal of fluorescence · 2017Article
- Biochemical and biophysical investigations of the interaction between human glucokinase and pro-apoptotic BAD.PloS one · 2017Article
- Solution NMR Spectroscopy for the Study of Enzyme Allostery.Chemical reviews · 2016Review
- Using NMR spectroscopy to elucidate the role of molecular motions in enzyme function.Progress in nuclear magnetic resonance spectroscopy · 2016Review
- Dual allosteric activation mechanisms in monomeric human glucokinase.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Kinetic Cooperativity in Human Pancreatic Glucokinase Originates from Millisecond Dynamics of the Small Domain.Angewandte Chemie (International ed. in English) · 2015Article
- Conformational heterogeneity and intrinsic disorder in enzyme regulation: Glucokinase as a case study.Intrinsically disordered proteins · 2015Article
- Role of connecting loop I in catalysis and allosteric regulation of human glucokinase.Protein science : a publication of the Protein Society · 2014Article
- Analysis of the co-operative interaction between the allosterically regulated proteins GK and GKRP using tryptophan fluorescence.The Biochemical journal · 2014Article
- A phospho-BAD BH3 helix activates glucokinase by a mechanism distinct from that of allosteric activators.Nature structural & molecular biology · 2014Article
- SUMOylation of pancreatic glucokinase regulates its cellular stability and activity.The Journal of biological chemistry · 2013Article
- GCK-MODY diabetes associated with protein misfolding, cellular self-association and degradation.Biochimica et biophysica acta · 2012Article
- Cooperativity in monomeric enzymes with single ligand-binding sites.Bioorganic chemistry · 2012Review
- Thermal stability of glucokinase (GK) as influenced by the substrate glucose, an allosteric glucokinase activator drug (GKA) and the osmolytes glycerol and urea.Biochimica et biophysica acta · 2012Article
- Insights into mechanism of glucokinase activation: observation of multiple distinct protein conformations.The Journal of biological chemistry · 2012Article
- Homotropic allosteric regulation in monomeric mammalian glucokinase.Archives of biochemistry and biophysics · 2012Review
- Order-disorder transitions govern kinetic cooperativity and allostery of monomeric human glucokinase.PLoS biology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
High-resolution nuclear magnetic resonance is used to investigate the conformational dynamics of human glucokinase, a 52 kDa monomeric enzyme that displays kinetic cooperativity. (1)H-(15)N transverse relaxation optimized spectra of uniformly labeled glucokinase, recorded in the absence and presence of glucose, reveal significant cross-peak overlap and heterogeneous peak intensities that persist over a range of temperatures. (15)N-specific labeling of isoleucines and tryptophans, reporting on backbone and side chain dynamics, respectively, demonstrates that both unliganded and glucose-bound enzymes sample multiple conformations, although glucose stabilizes certain conformations. These results provide the first direct evidence of glucokinase conformational heterogeneity and hence shed light on the molecular basis of cooperativity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.