ArticleAntimicrobial agents and chemotherapy2010
vanM, a new glycopeptide resistance gene cluster found in Enterococcus faecium.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed, 203 citations in OpenAlex.
- Optimizing a culture-enriched hybrid metagenomics pipeline to assess the AMR footprint of livestock manure in anaerobic digestate.Microbiology spectrum · 2026Article
- The interplay of specialized metabolites, antibiotic resistance, and virulence in Enterococcus faecium: in silico analysis of bacterial genomes.BMC genomics · 2026Article
- Molecular epidemiology of vancomycin resistant enterococci collected from 9 hospitals in Beijing, China: a multicenter study.Frontiers in cellular and infection microbiology · 2026Article
- Genomic characterization of vancomycin-resistantFrontiers in microbiology · 2026Article
- Rapid prediction of vancomycin-resistantFrontiers in microbiology · 2026Article
- High-resolution genomic and molecular characterization of vancomycin-resistant enterococci from hospitalized patients in a tertiary care center in Riyadh, Saudi Arabia.Scientific reports · 2025Article
- Genomic Characterization of Multiple Antibiotic-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Antimicrobial resistance and virulence potential of non-faecium and non-faecalis enterococci isolated from canine rectal swabs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Overexpression, Purification, and Biochemical Characterization of the vanC2 d-Ala-d-Ser Ligase fromACS omega · 2025Article
- Sludge amended soil induced multidrug and heavy metal resistance in endophytic Exiguobacterium sp. E21L: genomics evidences.World journal of microbiology & biotechnology · 2025Article
- Discovery of amino acid substitutions in penicillin-binding proteins associated with adaptation to D-Ala-D-Lac in vancomycin-resistantFrontiers in cellular and infection microbiology · 2025Article
- Molecular Characterization of Linezolid-Non-SusceptibleInfection and drug resistance · 2025Article
- Histidine kinase-mediated cross-regulation of the vancomycin-resistance operon in Clostridioides difficile.Molecular microbiology · 2024Article
- Vancomycin heteroresistance caused by unstable tandem amplifications of theAntimicrobial agents and chemotherapy · 2024Article
- Occurrence and Removal of Antibiotic Resistance in Nationwide Hospital Wastewater Deciphered by Metagenomics Approach - China, 2018-2022.China CDC weekly · 2023Article
- Determination of the Prevalence and Antimicrobial Resistance ofAntibiotics (Basel, Switzerland) · 2023Article
- Review
- Emergence of Clinical Clostridioides difficile Isolates With Decreased Susceptibility to Vancomycin.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Regulation of Resistance in Vancomycin-Resistant Enterococci: The VanRS Two-Component System.Microorganisms · 2021Review
- Genomic Sequence Analysis of Methicillin- and Carbapenem-Resistant Bacteria Isolated from Raw Sewage.Microbiology spectrum · 2021Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since glycopeptide-resistant enterococci (GRE) were reported in 1988, they have appeared in hospitals worldwide. Seven van gene cluster types (vanA, vanB, vanC, vanD, vanE, vanG, and vanL) are currently known. We investigated a clinical strain of Enterococcus faecium Efm-HS0661 that was isolated in 2006 from an inpatient with intra-abdominal infection in Shanghai. It was resistant to most antimicrobials, including vancomycin (MIC, >256 μg/ml) and teicoplanin (MIC, 96 μg/ml). Glycopeptide resistance could be transferred to E. faecium BM4105RF by conjugation. The donor and its transconjugant were negative by PCR for the known van genes. By cloning and primer walk sequencing, we discovered a novel van gene cluster, designated vanM. The vanM ligase gene was 1,032-bp in length and encoded a 343-amino-acid protein that shared 79.9, 70.8, 66.3, and 78.8% amino acid identity with VanA, VanB, VanD, and VanF, respectively. Although the vanM DNA sequence was closest to vanA, the organization of the vanM gene cluster was most similar to that of vanD. Upstream from the vanM cluster was an IS1216-like element, which may play a role in the dissemination of this resistance determinant. Liquid chromatography-mass spectrometry analysis of peptidoglycan precursors extracted from the VanM-type strain Efm-HS0661 treated with vancomycin or teicoplanin revealed a modified precursor (UDP-N-acetylmuramic acid [MurNAc]-tetrapeptide-D-Lac), indicating that VanM, like VanA, confers glycopeptide resistance by the inducible synthesis of precursor ending in D-Ala-D-Lac.
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