ArticleAddiction biology2011
Cell-specific effects of variants of the 68-base pair tandem repeat on prodynorphin gene promoter activity.
Article in Addiction biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Genetics of methamphetamine use disorder: A systematic review and meta-analyses of gene association studies.Neuroscience and biobehavioral reviews · 2021Pooled it
- Moderation of buprenorphine therapy for cocaine dependence efficacy by variation of the Prodynorphin gene.European journal of clinical pharmacology · 2022Trial
- Variants of opioid genes and response to treatment of opioid use disorder with buprenorphine-naloxone versus extended-release naltrexone in Caucasians.The American journal of drug and alcohol abuse · 2020Trial
- Opioid Use Disorders: Prevention, Diagnosis, and Treatment Strategies to Mitigate Addiction Risks.Current drug safety · 2025Review
- Epigenetic and Transcriptional Control of the Opioid Prodynorphine Gene: In-Depth Analysis in the Human Brain.Molecules (Basel, Switzerland) · 2021Review
- Current status of opioid addiction treatment and related preclinical research.Science advances · 2019Review
- Association of variants of prodynorphin promoter 68-bp repeats in caucasians with opioid dependence diagnosis: Effect on age trajectory of heroin use.Neuroscience letters · 2019Article
- Neuronal Expression of Opioid Gene is Controlled by Dual Epigenetic and Transcriptional Mechanism in Human Brain.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population.Molecular biology research communications · 2018Article
- Gender-specific association of functionalNeuropsychiatric disease and treatment · 2018Article
- Association between VNTR Polymorphism in Promoter Region of Prodynorphin (PDYN) Gene and Methamphetamine Dependence.Open access Macedonian journal of medical sciences · 2015Article
- A functional polymorphism in the prodynorphin gene affects cognitive flexibility and brain activation during reversal learning.Frontiers in behavioral neuroscience · 2015Article
- Genetics of opiate addiction.Current psychiatry reports · 2014Review
- Polymorphisms of the kappa opioid receptor and prodynorphin genes: HIV risk and HIV natural history.Journal of acquired immune deficiency syndromes (1999) · 2013Article
- κ-opioid receptor/dynorphin system: genetic and pharmacotherapeutic implications for addiction.Trends in neurosciences · 2012Review
- The genetics of the opioid system and specific drug addictions.Human genetics · 2012Review
- Genetic association analyses of PDYN polymorphisms with heroin and cocaine addiction.Genes, brain, and behavior · 2012Article
- The dynorphin/κ-opioid receptor system and its role in psychiatric disorders.Cellular and molecular life sciences : CMLS · 2012Review
- Tissue-specific DNA methylation of the human prodynorphin gene in post-mortem brain tissues and PBMCs.Pharmacogenetics and genomics · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A polymorphic 68-bp tandem repeat has been identified within the promoter of the human prodynorphin (PDYN) gene. We found that this 68-bp repeat in the PDYN promoter occurs naturally up to five times. We studied the effect of the number of 68-bp repeats, and of a SNP (rs61761346) found within the repeat on PDYN gene promoter activity. Thirteen promoter forms, different naturally occurring combinations of repeats and the internal SNP, were cloned upstream of the luciferase reporter gene, transfected into human SK-N-SH, H69, or HEK293 cells. Cells were then stimulated with TPA or caffeine. We found cell-specific effects of the number of 68-bp repeats on the transcriptional activity of the PDYN promoter. In SK-N-SH and H69 cells, three or four repeats led to lower expression of luciferase than did one or two repeats. The opposite effect was found in HEK293 cells. The SNP also had an effect on PDYN gene expression in both SK-N-SH and H69 cells; promoter forms with the A allele had significantly higher expression than promoter forms with the G allele. These results further our understanding of the complex transcriptional regulation of the PDYN gene promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.