ArticleNature methods2010
An antibiotic selection marker for nematode transgenesis.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 72 citations in OpenAlex.
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- Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.Current protocols · 2023Article
- Highly efficient transgenesis with miniMos in Caenorhabditis briggsae.G3 (Bethesda, Md.) · 2022Article
- Mutagenesis and structural modeling implicate RME-8 IWN domains as conformational control points.PLoS genetics · 2022Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Megapixel camera arrays enable high-resolution animal tracking in multiwell plates.Communications biology · 2022Article
- Floxed exon (Flexon): A flexibly positioned stop cassette for recombinase-mediated conditional gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.Cell reports · 2021Article
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- A histamine-gated channel is an efficient negative selection marker formicroPublication biology · 2021Article
- Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations inG3 (Bethesda, Md.) · 2020Article
- Characterizing a strong pan-muscular promoter (PmicroPublication biology · 2020Article
- Genetic and epigenetic effects on centromere establishment.Chromosoma · 2020Review
- Tools formicroPublication biology · 2019Article
- TheGenetics · 2019Review
- Purine Homeostasis Is Necessary for Developmental Timing, Germline Maintenance and Muscle Integrity inGenetics · 2019Article
- Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.Antimicrobial agents and chemotherapy · 2018Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.