ArticleNature methods2010
Rapid selection of transgenic C. elegans using antibiotic resistance.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- Screening conditions and constructs for attempted genetic transformation of C. elegans by Agrobacterium.PloS one · 2025Article
- FLInt: single shot safe harbor transgene integration via Fluorescent Landmark Interference.G3 (Bethesda, Md.) · 2023Article
- Neuronal HLH-30/TFEB modulates peripheral mitochondrial fragmentation to improve thermoresistance in Caenorhabditis elegans.Aging cell · 2023Article
- Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.Current protocols · 2023Article
- Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster.Current protocols · 2023Article
- Article
- Highly efficient transgenesis with miniMos in Caenorhabditis briggsae.G3 (Bethesda, Md.) · 2022Article
- Benzimidazoles cause lethality by inhibiting the function of Caenorhabditis elegans neuronal beta-tubulin.International journal for parasitology. Drugs and drug resistance · 2022Article
- Mutagenesis and structural modeling implicate RME-8 IWN domains as conformational control points.PLoS genetics · 2022Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Megapixel camera arrays enable high-resolution animal tracking in multiwell plates.Communications biology · 2022Article
- Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.Cell reports · 2021Article
- Article
- A histamine-gated channel is an efficient negative selection marker formicroPublication biology · 2021Article
- Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations inG3 (Bethesda, Md.) · 2020Article
- Genetic and epigenetic effects on centromere establishment.Chromosoma · 2020Review
- Curative Treatment of Candidiasis by the Live Biotherapeutic MicroorganismMicroorganisms · 2019Article
- Tools formicroPublication biology · 2019Article
- TheGenetics · 2019Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.