ArticleProceedings of the National Academy of Sciences of the United States of America2010
Conserved microRNA targeting in Drosophila is as widespread in coding regions as in 3'UTRs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 153 citations in OpenAlex.
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- Biochemical principles of miRNA targeting in flies.Nature communications · 2026Article
- A time course analysis through diapause reveals dynamic temporal patterns of microRNAs associated with endocrine regulation in the butterfly Pieris napi.Molecular ecology · 2025Article
- Dicer Is Involved in Cytotoxicity and Motor Impairment Induced by TBPH Deficiency.Current issues in molecular biology · 2025Article
- Exosome-shuttled miR-5121 from A2 astrocytes promotes BSCB repair after traumatic SCI by activating autophagy in vascular endothelial cells.Journal of nanobiotechnology · 2025Article
- Analysis ofbioRxiv : the preprint server for biology · 2024Article
- The circular RNA circATP8B(2) regulates ROS production and antiviral immunity in Drosophila.Cell reports · 2024Article
- A tailed mirtron promotes longevity in Drosophila.Nucleic acids research · 2024Article
- Analysis ofFrontiers in cellular and infection microbiology · 2024Article
- Rocket-miR, a translational launchpad for miRNA-based antimicrobial drug development.mSystems · 2023Article
- In silico methods for predicting functional synonymous variants.Genome biology · 2023Review
- Identification of MicroRNAs in the West Nile Virus Vector Culex tarsalis (Diptera: Culicidae).Journal of medical entomology · 2023Article
- Transcriptome-Wide Analysis of microRNA-mRNA Correlations in Tissue Identifies microRNA Targeting Determinants.Non-coding RNA · 2023Article
- circRNA432 enhances the coelomocyte phagocytosis via regulating the miR-2008-ELMO1 axis in Vibrio splendidus-challenged Apostichopus japonicus.Communications biology · 2023Article
- MicroRNAs reshape the immunity of insects in response to bacterial infection.Frontiers in immunology · 2023Review
- Synonymous Variants: Necessary Nuance in Our Understanding of Cancer Drivers and Treatment Outcomes.Journal of the National Cancer Institute · 2022Review
- Learning noncoding RNA biology from viruses.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Identification of microRNAs in the Lyme Disease VectorInternational journal of molecular sciences · 2022Article
- LncRNA AZIN1-AS1 ameliorates myocardial ischemia-reperfusion injury by targeting miR-6838-5p/WNT3A axis to activate Wnt-β/catenin signaling pathway.In vitro cellular & developmental biology. Animal · 2022Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are a class of short noncoding RNAs that regulate protein-coding genes posttranscriptionally. In animals, most known miRNA targeting occurs within the 3'UTR of mRNAs, but the extent of biologically relevant targeting in the ORF or 5'UTR of mRNAs remains unknown. Here, we develop an algorithm (MinoTar-miRNA ORF Targets) to identify conserved regulatory motifs within protein-coding regions and use it to estimate the number of preferentially conserved miRNA-target sites in ORFs. We show that, in Drosophila, preferentially conserved miRNA targeting in ORFs is as widespread as it is in 3'UTRs and that, while far less abundant, conserved targets in Drosophila 5'UTRs number in the hundreds. Using our algorithm, we predicted a set of high-confidence ORF targets and selected seven miRNA-target pairs from among these for experimental validation. We observed down-regulation by the miRNA in five out of seven cases, indicating our approach can recover functional sites with high confidence. Additionally, we observed additive targeting by multiple sites within a single ORF. Altogether, our results demonstrate that the scale of biologically important miRNA targeting in ORFs is extensive and that computational tools such as ours can aid in the identification of such targets. Further evidence suggests that our results extend to mammals, but that the extent of ORF and 5'UTR targeting relative to 3'UTR targeting may be greater in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.