ArticleHuman reproduction (Oxford, England)2010
In vitro developmental potential of macaque oocytes, derived from unstimulated ovaries, following maturation in the presence of glutathione ethyl ester.
Article in Human reproduction (Oxford, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Decidual Stromal Cell Ferroptosis Associated with Abnormal Iron Metabolism Is Implicated in the Pathogenesis of Recurrent Pregnancy Loss.International journal of molecular sciences · 2023Article
- Meiosis Resumption of Immature Human Oocytes following Treatment with Calcium IonophoreCell journal · 2021Article
- Ceratonia siliqua (Carob) extract improved in vitro development of vitrified-warmed mouse germinal vesicle oocytes: assessment of possible mechanism.Cell and tissue banking · 2021Article
- Article
- Preincubation with glutathione ethyl ester improves the developmental competence of vitrified mouse oocytes.Journal of assisted reproduction and genetics · 2018Article
- Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes.Human reproduction (Oxford, England) · 2016Article
- The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study.Clinical and experimental reproductive medicine · 2015Article
- In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes.PloS one · 2015Article
- Expression of a2 vacuolar ATPase in spermatozoa is associated with semen quality and chemokine-cytokine profiles in infertile men.PloS one · 2013Article
- The effect of human cumulus cells on the maturation and developmental potential of immature oocytes in ICSI cycles.Journal of assisted reproduction and genetics · 2012Article
- Induced pluripotent stem cells from highly endangered species.Nature methods · 2011Article
- Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome.American journal of physiology. Endocrinology and metabolism · 2011Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe inadequacies of oocyte in vitro maturation (IVM) systems for both non-human primates and humans are evidenced by reduced fertilization and poor embryonic development, and may be partly explained by significantly lower glutathione (GSH) contents compared with in vivo matured (IVO) oocytes. As this influence has not been fully explored, this study investigated the effect of the GSH donor, glutathione ethyl ester (GSH-OEt), on the IVM and development of macaque oocytes as a model of human oocyte IVM.
methodsMacaque oocytes derived from unstimulated ovaries were cultured in mCMRL-1066 alone or supplemented with 3 or 5 mM GSH-OEt. In vitro matured oocytes were subjected to the GSH assay, fixed for the assessment of spindle morphology or prepared ICSI. Embryo development of zygotes cultured in mHECM-9 was assessed up to Day 9 post-ICSI. RESULTS Supplementation of the maturation medium with GSH-OEt significantly increased oocyte maturation and normal fertilization rates compared with control oocytes, but only 5 mM GSH-OEt significantly increased the oocyte and cumulus cell GSH content. Confocal microscopy revealed significant differences in the spindle morphology between IVO and control in vitro matured metaphase II oocytes. Oocytes matured with 5 mM GSH-OEt exhibited spindle area and spindle pole width similar to that seen in the IVO oocyte. While no significant differences were observed in blastocyst rates, addition of 3 mM GSH-OEt during IVM significantly increased the proportion of embryos developing to the 5-8 cell stage while 5 mM GSH-OEt significantly increased the proportion of morula-stage embryos compared with controls.
conclusionsSupplementation of the IVM medium with GSH-OEt promotes better maturation and normal fertilization of macaque oocytes compared with non-supplemented medium. However, further improvement of the primate oocyte IVM culture system is required to support better blastocyst development of oocytes derived from unstimulated ovaries.
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