ArticleThe Journal of biological chemistry2010
Binding of Toxoplasma gondii glycosylphosphatidylinositols to galectin-3 is required for their recognition by macrophages.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- From conventional to biosensor-based detection ofFood and waterborne parasitology · 2026Review
- Free GPIs and Comparison of GPI Structures Among Species.International journal of molecular sciences · 2025Review
- The Role of Glycans in Human Immunity-A Sweet Code.Molecules (Basel, Switzerland) · 2025Review
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- Article
- The GPI sidechain ofmBio · 2024Article
- Galectin-3 plays a key role in controlling infection byFrontiers in cellular and infection microbiology · 2024Article
- (Patho)Physiology of Glycosylphosphatidylinositol-Anchored Proteins I: Localization at Plasma Membranes and Extracellular Compartments.Biomolecules · 2023Review
- The importance of side branches of glycosylphosphatidylinositol anchors: a molecular dynamics perspective.Glycobiology · 2022Article
- Galectin-3, a rising star in modulating microglia activation under conditions of neurodegeneration.Cell death & disease · 2022Review
- Lipid profile of Trichinella papuae muscle-stage larvae.Scientific reports · 2020Article
- Babesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2-biased cytokine profiles in antigen presenting cells.Biochimie · 2019Article
- CRISPR/Cas9 and glycomics tools forThe Journal of biological chemistry · 2019Article
- LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation.The EMBO journal · 2018Article
- The roles of galectins in parasitic infections.Acta tropica · 2018Review
- Key regulators of galectin-glycan interactions.Proteomics · 2016Review
- Innate immunity against molecular mimicry: Examining galectin-mediated antimicrobial activity.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Possible role of TLR4 and TLR9 SNPs in protection against congenital toxoplasmosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Evolving mechanistic insights into galectin functions.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Internalization and TLR-dependent type I interferon production by monocytes in response to Toxoplasma gondii.Immunology and cell biology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We showed that the production of tumor necrosis factor (TNF) α by macrophages in response to Toxoplasma gondii glycosylphosphatidylinositols (GPIs) requires the expression of both Toll-like receptors TLR2 and TLR4, but not of their co-receptor CD14. Galectin-3 is a β-galactoside-binding protein with immune-regulatory effects, which associates with TLR2. We demonstrate here by using the surface plasmon resonance method that the GPIs of T. gondii bind to human galectin-3 with strong affinity and in a dose-dependent manner. The use of a synthetic glycan and of the lipid moiety cleaved from the GPIs shows that both parts are involved in the interaction with galectin-3. GPIs of T. gondii also bind to galectin-1 but with a lower affinity and only through the lipid moiety. At the cellular level, the production of TNF-α induced by T. gondii GPIs in macrophages depends on the expression of galectin-3 but not of galectin-1. This study is the first identification of a galectin-3 ligand of T. gondii origin, and galectin-3 might be a co-receptor presenting the GPIs to the TLRs on macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.