ArticleActa biomaterialia2011
Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds.
Article in Acta biomaterialia, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 115 citations in OpenAlex.
- Design Requirements for Annulus Fibrosus Repair: Review of Forces, Displacements, and Material Properties of the Intervertebral Disk and a Summary of Candidate Hydrogels for Repair.Journal of biomechanical engineering · 2016Pooled it
- Characterization of Hydrolytic and Thermomechanical Stability of 3D-Printed PLDLA-TMC 60/40 Scaffolds for Cartilage Tissue Applications.ACS omega · 2026Article
- Fabrication and characterization of novel PLA/CH/GelMA/hydroxyapatite electrospun nanofibers for bone tissue regeneration.RSC advances · 2026Article
- Topology-optimized melt-electrowritten PCL patch for abdominal wall reconstruction.Bioactive materials · 2026Article
- Elastic fibers define embryonic tissue stiffness to enable buckling morphogenesis of the small intestine.Biomaterials · 2023Article
- "Slow walk" mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel.Bioactive materials · 2023Article
- Textile Design of an Intervertebral Disc Replacement Device from Silk Yarn.Biomimetics (Basel, Switzerland) · 2023Article
- Article
- Enthesis Healing Is Dependent on Scaffold Interphase Morphology-Results from a Rodent Patellar Model.Cells · 2022Article
- The role of microenvironment in stem cell-based regeneration of intervertebral disc.Frontiers in bioengineering and biotechnology · 2022Review
- Demineralized Cortical Bone Matrix Augmented With Peripheral Blood-Derived Mesenchymal Stem Cells for Rabbit Medial Meniscal Reconstruction.Frontiers in bioengineering and biotechnology · 2022Article
- Article
- Cytotoxicity analysis of biomass combustion particles in human pulmonary alveolar epithelial cells on an air-liquid interface/dynamic culture platform.Particle and fibre toxicology · 2021Article
- Putting the Pieces in Place: Mobilizing Cellular Players to Improve Annulus Fibrosus Repair.Tissue engineering. Part B, Reviews · 2021Review
- Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes.Science advances · 2021Article
- Electrospun Fiber Alignment Guides Osteogenesis and Matrix Organization Differentially in Two Different Osteogenic Cell Types.Frontiers in bioengineering and biotechnology · 2021Article
- Success Criteria and Preclinical Testing of Multifunctional Hydrogels for Tendon Regeneration.Tissue engineering. Part C, Methods · 2020Article
- Crosslinker concentration controls TGFβ-3 release and annulus fibrosus cell apoptosis in genipin-crosslinked fibrin hydrogels.European cells & materials · 2020Article
- Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.International journal of nanomedicine · 2020Article
- Disordered Mechanical Stress and Tissue Engineering Therapies in Intervertebral Disc Degeneration.Polymers · 2019Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Electrospun nanofibrous scaffolds have become widely investigated for tissue engineering applications, owing to their ability to replicate the scale and organization of many fiber-reinforced soft tissues such as the knee meniscus, the annulus fibrosus of the intervertebral disc, tendon, and cartilage. However, due to their small pore size and dense packing of fibers, cellular ingress into electrospun scaffolds is limited. Progress in the application of electrospun scaffolds has therefore been hampered, as limited cell infiltration results in heterogeneous deposition of extracellular matrix and mechanical properties that remain below native benchmarks. In the present study, dynamic culture conditions dramatically improved the infiltration of mesenchymal stem cells into aligned nanofibrous scaffolds. While dynamic culture resulted in a reduction of glycosaminoglycan content, removal from dynamic culture to free-swelling conditions after 6 weeks resulted recovery of glycosaminoglycan content. Dynamic culture significantly increased collagen content, and collagen was more uniformly distributed throughout the scaffold thickness. While mechanical function was assessed and tensile modulus increased with culture duration, dynamic culture did not result in any additional improvement beyond free-swelling culture. Transient dynamic (6 weeks dynamic followed by 6 weeks free-swelling) culture significantly enhanced cell infiltration while permitting GAG accumulation. In this study, we demonstrated that a simple modification to standard in vitro culture conditions effectively improves cellular ingress into electrospun scaffolds, resolving a challenge which has until now limited the utility of these materials for various tissue engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.