Evidence map›Paper›PMID 20728441›Full record

ArticleFEBS letters2010

Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase.

Marie E Maradeo, Robert V Skibbens

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Marie E MaradeoDepartment of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA.
Robert V Skibbens
Lehigh University · US

Funding

Mechanisms of Sister Chromatid PairingR15GM083269 · NIGMS · LEHIGH UNIVERSITY · PI SKIBBENS, ROBERT · 2008 to 2011
$522k
NIGMS NIH HHS 1R15GM083269NIGMS NIH HHS R15 GM083269
6 · The paper itself

Abstract

Ctf7/Eco1-dependent acetylation of Smc3 is essential for sister chromatid cohesion. Here, we use epitope tag-induced lethality in cells diminished for Ctf7/Eco1 activity to map cohesin architecture in vivo. Tagging either Smc1 or Mcd1/Scc1, but not Scc3/Irr1, appears to abolish access to Smc3 in ctf7/eco1 mutant cells, suggesting that Smc1 and Smc3 head domains are in direct contact with each other and also with Mcd1/Scc1. Thus, cohesin complexes may be much more compact than commonly portrayed. We further demonstrate that mutation in ELG1 or RFC5 anti-establishment genes suppress tag-induced lethality, consistent with the notion that the replication fork regulates Ctf7/Eco1.

Indexed as

AcetylationAcetyltransferasesCarrier ProteinsCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsNuclear ProteinsProtein ConformationProtein Interaction MappingProtein MultimerizationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsStructural Maintenance of Chromosome Protein 1AcetyltransferasesCarrier ProteinsCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsECO1 protein, S cerevisiaeElg1 protein, S cerevisiaeIRR1 protein, S cerevisiaeMCD1 protein, S cerevisiaeNuclear ProteinsSaccharomyces cerevisiae ProteinsStructural Maintenance of Chromosome Protein 1

Identifiers

PMID20728441
PMCPMC2946494
OpenAlexW2142779113

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.