Evidence map›Paper›PMID 20727848›Full record

ReviewBiochimica et biophysica acta2011

Comparative NMR studies demonstrate profound differences between two viroporins: p7 of HCV and Vpu of HIV-1.

Gabriel A Cook, Hua Zhang, Sang Ho Park, Yan Wang, Stanley J Opella

Abstract readComparative StudyReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Biochimica et biophysica acta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Targeting the Channel Activity of Viroporins.Advances in protein chemistry and structural biology · 2016
    Review
  5. Article
  6. Review
  7. Review
  8. Magic angle spinning NMR of viruses.Progress in nuclear magnetic resonance spectroscopy · 2015
    Review
  9. Article
  10. Article
  11. When detergent meets bilayer: birth and coming of age of lipid bicelles.Progress in nuclear magnetic resonance spectroscopy · 2013
    Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Gabriel A CookDepartment of Chemistry and Biochemistry, University of California, San Diego, Lajolla, CA 92093, USA.
Hua Zhang
Sang Ho Park
Yan Wang
Stanley J Opella
University of California San Diego · US

Funding

RESOURCE FOR SOLID STATE NMR OF PROTEINSP41EB002031 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2003 to 2018
$16.3M
Molecular Imaging of G-Protein-Coupled Receptors for Drug DevelopmentR01EB005161 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2006 to 2014
$6.8M
Structure, Dynamics, and Functions of the Viroporins Vpu from HIV and p7 from HCVR01GM066978 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2003 to 2016
$3.5M
Membrane Protein Structures:Phospholipid Bilayers (RMI)R01GM075877 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2005 to 2009
$1.4M
NIBIB NIH HHS P41 EB002031NIBIB NIH HHS R01 EB005161NIGMS NIH HHS R01 GM066978NIGMS NIH HHS R01 GM075877
6 · The paper itself

Abstract

The p7 protein from hepatitis C virus and the Vpu protein from HIV-1 are members of the viroporin family of small viral membrane proteins. It is essential to determine their structures in order to obtain an understanding of their molecular mechanisms and to develop new classes of anti-viral drugs. Because they are membrane proteins, it is challenging to study them in their native phospholipid bilayer environments by most experimental methods. Here we describe applications of NMR spectroscopy to both p7 and Vpu. Isotopically labeled p7 and Vpu samples were prepared by heterologous expression in bacteria, initial isolation as fusion proteins, and final purification by chromatography. The purified proteins were studied in the model membrane environments of micelles by solution NMR spectroscopy and in aligned phospholipid bilayers by solid-state NMR spectroscopy. The resulting structural findings enable comparisons to be made between the two proteins, demonstrating that they have quite different architectures. Most notably, Vpu has one trans-membrane helix and p7 has two trans-membrane helices; in addition, there are significant differences in the structures and dynamics of their internal loop and terminal regions.

Indexed as

HepacivirusHIV-1Human Immunodeficiency Virus ProteinsIon ChannelsLipid BilayersMicellesModels, MolecularNuclear Magnetic Resonance, BiomolecularPorinsRecombinant Fusion ProteinsViral ProteinsViral Regulatory and Accessory ProteinsViroporin ProteinsHuman Immunodeficiency Virus ProteinsIon ChannelsLipid BilayersMicellesp7 protein, Hepatitis C virusPorinsRecombinant Fusion ProteinsViral ProteinsViral Regulatory and Accessory ProteinsViroporin Proteinsvpu protein, Human immunodeficiency virus 1

Identifiers

PMID20727848
PMCPMC3005007
OpenAlexW2161488169

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.