ArticleJournal of biomedical science2010
Reactive oxygen species are involved in regulating alpha1-adrenoceptor-activated vascular smooth muscle contraction.
Article in Journal of biomedical science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
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- Impact of High-Altitude Hypoxia on Bone Defect Repair: A Review of Molecular Mechanisms and Therapeutic Implications.Frontiers in medicine · 2022Review
- Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects.Frontiers in physiology · 2022Article
- 17β-Estradiol Treatment Improves Acetylcholine-Induced Relaxation of Mesenteric Arteries in Ovariectomized UC Davis Type 2 Diabetes Mellitus Rats in Prediabetic State.Frontiers in physiology · 2022Article
- Avocado Oil Prevents Kidney Injury and Normalizes Renal Vasodilation after Adrenergic Stimulation in Hypertensive Rats: Probable Role of Improvement in Mitochondrial Dysfunction and Oxidative Stress.Life (Basel, Switzerland) · 2021Article
- Reduced Endothelial Leptin Signaling Increases Vascular Adrenergic Reactivity in a Mouse Model of Congenital Generalized Lipodystrophy.International journal of molecular sciences · 2021Article
- Inhibition of NOX1 Mitigates Blood Pressure Increases in Elastin Insufficiency.Function (Oxford, England) · 2021Article
- Selective Blockade of TNFR1 Improves Clinical Disease and Bronchoconstriction in Experimental RSV Infection.Viruses · 2020Article
- The Role of Glycocalyx and Caveolae in Vascular Homeostasis and Diseases.Frontiers in physiology · 2020Review
- Nox2 contributes to hyperinsulinemia-induced redox imbalance and impaired vascular function.Redox biology · 2017Article
- Lidocaine relaxation in isolated rat aortic rings is enhanced by endothelial removal: possible role of KBMC anesthesiology · 2016Article
- Efficacy of ellagic acid and sildenafil in diabetes-induced sexual dysfunction.Pharmacognosy magazine · 2014Article
- The fate of the primary cilium during myofibroblast transition.Molecular biology of the cell · 2014Article
- Inhibition of As(III) and Hg(II) caused aortic hypercontraction by eugenol, linalool and carvone.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2014Article
- Reactive oxygen species are involved in regulating hypocontractility of mesenteric artery to norepinephrine in cirrhotic rats with portal hypertension.International journal of biological sciences · 2014Article
- Selective inhibition of NADPH oxidase reverses the over contraction of diabetic rat aorta.Redox biology · 2014Article
- Redox signaling in pathophysiology of hypertension.Journal of biomedical science · 2013Review
- Ethers and esters derived from apocynin avoid the interaction between p47phox and p22phox subunits of NADPH oxidase: evaluation in vitro and in silico.Bioscience reports · 2013Article
- Current status of NADPH oxidase research in cardiovascular pharmacology.Vascular health and risk management · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReactive oxygen species (ROS) were shown to mediate aberrant contractility in hypertension, yet the physiological roles of ROS in vascular smooth muscle contraction have remained elusive. This study aimed to examine whether ROS regulate alpha1-adrenoceptor-activated contraction by altering myosin phosphatase activities.
methodsUsing endothelium-denuded rat tail artery (RTA) strips, effects of anti-oxidants on isometric force, ROS production, phosphorylation of the 20-kDa myosin light chain (MLC20), and myosin phosphatase stimulated by alpha1-adrenoceptor agonist phenylephrine were examined.
resultsAn antioxidant, N-acetyl-L-cysteine (NAC), and two NADPH oxidase inhibitors, apocynin and VAS2870, dose-dependently inhibited contraction activated by phenylephrine. Phenylephrine stimulated superoxide anion production that was diminished by the pretreatment of apocynin, VAS2870, superoxide scavenger tiron or mitochondria inhibitor rotenone, but not by xanthine oxidase inhibitor allopurinol or cyclooxygenase inhibitor indomethacin. Concurrently, NADPH oxidase activity in RTA homogenates increased within 1 min upon phenylephrine stimulation, sustained for 10 min, and was abolished by the co-treatment with apocynin, but not allopurinol or rotenone. Phenylephrine-induced MLC20 phosphorylation was dose-dependently decreased by apocynin. Furthermore, apocynin inhibited phenylephrine-stimulated RhoA translocation to plasma membrane and phosphorylation of both myosin phosphatase regulatory subunit MYPT1Thr855 and myosin phosphatase inhibitor CPI-17Thr38.
conclusionsROS, probably derived from NADPH oxidase and mitochondria, partially regulate alpha1-adrenoceptor-activated smooth muscle contraction by altering myosin phosphatase-mediated MLC20 phosphorylation through both RhoA/Rho kinase- and CPI-17-dependent pathways.
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