ArticleTraffic (Copenhagen, Denmark)2010
HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components.
Article in Traffic (Copenhagen, Denmark), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
58 citing papers in PubMed, 95 citations in OpenAlex.
- Article
- Article
- Probing Gag-Env dynamics at HIV-1 assembly sites using live-cell microscopy.Journal of virology · 2024Article
- Roles of RNA scaffolding in nanoscale Gag multimerization and selective protein sorting at HIV membranes.Science advances · 2024Article
- Methods for Engineering Binders to Multi-Pass Membrane Proteins.Bioengineering (Basel, Switzerland) · 2023Review
- HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.Nature communications · 2023Article
- Article
- Specific Interaction of DARPin with HIV-1 CAViruses · 2022Article
- Review
- Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein.Small GTPases · 2022Article
- CD82 and Gangliosides Tune CD81 Membrane Behavior.International journal of molecular sciences · 2021Article
- Review
- Review
- HIV Reactivation in Latently Infected Cells With Virological Synapse-Like Cell Contact.Viruses · 2020Article
- In-depth phenotypic characterization of reticulocyte maturation using mass cytometry.Blood cells, molecules & diseases · 2018Article
- How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins.Nature communications · 2018Article
- Tetraspanin Assemblies in Virus Infection.Frontiers in immunology · 2018Review
- Cholesterol Promotes Protein Binding by Affecting Membrane Electrostatics and Solvation Properties.Biophysical journal · 2017Article
- Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.Platelets · 2017Article
- The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 3 countries.
Funding
Abstract
Partitioning of membrane proteins into various types of microdomains is crucial for many cellular functions. Tetraspanin-enriched microdomains (TEMs) are a unique type of protein-based microdomain, clearly distinct from membrane rafts, and important for several cellular processes such as fusion, migration and signaling. Paradoxically, HIV-1 assembly/egress occurs at TEMs, yet the viral particles also incorporate raft lipids. Using different quantitative microscopy approaches, we investigated the dynamic relationship between TEMs, membrane rafts and HIV-1 exit sites, focusing mainly on the tetraspanin CD9. Our results show that clustering of CD9 correlates with multimerization of the major viral structural component, Gag, at the plasma membrane. CD9 exhibited confined behavior and reduced lateral mobility at viral assembly sites, suggesting that Gag locally traps tetraspanins. In contrast, the raft lipid GM1 and the raft-associated protein CD55, while also recruited to assembly/budding sites, were only transiently trapped in these membrane areas. CD9 recruitment and confinement were found to be partially dependent on cholesterol, while those of CD55 were completely dependent on cholesterol. Importantly, our findings support the emerging concept that cellular and viral components, instead of clustering at preexisting microdomain platforms, direct the formation of distinct domains for the execution of specific functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.