ArticleBritish journal of pharmacology2010
Caffeine inhibits the viability and osteogenic differentiation of rat bone marrow-derived mesenchymal stromal cells.
Article in British journal of pharmacology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Review
- Article
- Alveolar Bone Repair Dynamics in Rats Influenced by Coffee Ingestion: A Confocal Microscopy Analysis.Cureus · 2025Article
- Sodium butyrate protect bone mass in lipopolysaccharide-treated rats by reducing oxidative stress and inflammatory.Redox report : communications in free radical research · 2024Article
- Caffeine regulates both osteoclast and osteoblast differentiation via the AKT, NF-κB, and MAPK pathways.Frontiers in pharmacology · 2024Article
- Are we undertreating calcium deficiency in metabolic bone disease of prematurity? A case report and review.Frontiers in pediatrics · 2022Article
- Psoralidin prevents caffeine-induced damage and abnormal differentiation of bone marrow mesenchymal stem cells via the classical estrogen receptor pathway.Annals of translational medicine · 2021Article
- Bone cement as a local chemotherapeutic drug delivery carrier in orthopedic oncology: A review.Journal of bone oncology · 2021Review
- Does Drinking Coffee and Tea Affect Bone Metabolism in Patients with Inflammatory Bowel Diseases?Nutrients · 2021Review
- Is Impregnation of Xenograft with Caffeine Effective on Bone Healing Rate in Mandibular Defects? A Pilot Histological Animal Study.Journal of maxillofacial and oral surgery · 2020Article
- Association of Protein and Vitamin D Intake With Biochemical Markers in Premature Osteopenic Infants: A Case-Control Study.Frontiers in pediatrics · 2020Article
- Effect of Black Tea Extract and Thearubigins on Osteoporosis in Rats and Osteoclast FormationFrontiers in physiology · 2018Article
- Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy.BMC musculoskeletal disorders · 2015Article
- Lithium enhanced cell proliferation and differentiation of mesenchymal stem cells to neural cells in rat spinal cord.International journal of clinical and experimental pathology · 2015Article
- Parathyroid hormone as a marker for metabolic bone disease of prematurity.Journal of perinatology : official journal of the California Perinatal Association · 2014Observational
- A novel human TGF-β1 fusion protein in combination with rhBMP-2 increases chondro-osteogenic differentiation of bone marrow mesenchymal stem cells.International journal of molecular sciences · 2014Article
- Article
- In vitro effects of caffeine in growth cartilage of rats.Acta ortopedica brasileira · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeCaffeine is consumed extensively in Europe and North America. As a risk factor for osteoporosis, epidemiological studies have observed that caffeine can decrease bone mineral density, adversely affect calcium absorption and increase the risk of bone fracture. However, the exact mechanisms have not been fully investigated. Here, we examined the effects of caffeine on the viability and osteogenesis of rat bone marrow-derived mesenchymal stromal cells (rBMSCs). EXPERIMENTAL APPROACH: Cell viability, apoptosis and necrosis were quantified using thymidine incorporation and flow cytometry. Sequential gene expressions in osteogenic process were measured by real-time PCR. cAMP, alkaline phosphatase and osteocalcin were assessed by immunoassay, spectrophotometry and radioimmunoassay, respectively. Mineralization was determined by calcium deposition. KEY
resultsAfter treating BMSCs with high caffeine concentrations (0.1-1mM), their viability decreased in a concentration-dependent manner. This cell death was primarily due to necrosis and, to a small extent, apoptosis. Genes and protein sequentially expressed in osteogenesis, including Cbfa1/Runx2, collagen I, alkaline phosphatase and its protein, were significantly downregulated except for osteocalcin and its protein. Moreover, caffeine inhibited calcium deposition in a concentration- and time-dependent manner, but increased intracellular cAMP in a concentration-dependent manner. CONCLUSIONS AND IMPLICATIONS: By suppressing the commitment of BMSCs to the osteogenic lineage and selectively inhibiting gene expression, caffeine downregulated some important events in osteogenesis and ultimately affected bone mass.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.