ArticleJournal of nucleic acids2010
Preparation of DNA ladder based on multiplex PCR technique.
Article in Journal of nucleic acids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A Quantitative Description for Optical Mass Measurement of Single Biomolecules.ACS photonics · 2023Article
- "In-house" production of DNA size marker from a vaccinal Bacillus anthracis strain.Iranian journal of microbiology · 2015Article
- A novel combined method for cost-benefit production of DNA ladders.Advanced biomedical research · 2015Article
- Easy method for production of a home-made DNA ladder in every laboratory.Advanced biomedical research · 2015Article
- Straightforward procedure for laboratory production of DNA ladder.Journal of nucleic acids · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100-1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA. Target DNA fragments were amplified using Touchdown PCR combined with hot start PCR, respectively, followed extracted by phenol/chloroform, precipitated with ethanol and mixed thoroughly. The results showed that the 100-1000 bp DNA fragments were successfully obtained in one PCR reaction, the bands of prepared DNA marker were clear, the size was right and could be used as control in the molecular biology experiment. This method could save time and be more inexpensive, rapid, simple when compared with the current DNA Ladder prepared means.
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Registered trials
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