ArticleAmerican journal of respiratory cell and molecular biology2011
Human bronchial epithelial cells differentiate to 3D glandular acini on basement membrane matrix.
Article in American journal of respiratory cell and molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
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- Identification of a Novel Subset of Human Airway Epithelial Basal Stem Cells.International journal of molecular sciences · 2024Article
- Motile Living Biobots Self-Construct from Adult Human Somatic Progenitor Seed Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A roadmap for developing and engineeringBiophysics reviews · 2023Review
- Review
- Modeling Cell Communication in Cancer With Organoids: Making the Complex Simple.Frontiers in cell and developmental biology · 2020Review
- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Cancer-associated fibroblasts suppress SOX2-induced dysplasia in a lung squamous cancer coculture.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nasospheroids permit measurements of CFTR-dependent fluid transport.JCI insight · 2017Article
- [Advances in Classification and Research Methods of Lung Epithelial Stem and Progenitor Cells].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2017Review
- Organotypic culture in three dimensions prevents radiation-induced transformation in human lung epithelial cells.Scientific reports · 2016Article
- Review
- Development of glandular models from human nasal progenitor cells.American journal of respiratory cell and molecular biology · 2015Article
- Concise review: the relevance of human stem cell-derived organoid models for epithelial translational medicine.Stem cells (Dayton, Ohio) · 2013Review
- Article
- Isolation of basal cells and submucosal gland duct cells from mouse trachea.Journal of visualized experiments : JoVE · 2012Article
- Glandular gene expression of sinus mucosa in chronic rhinosinusitis with and without cystic fibrosis.American journal of respiratory cell and molecular biology · 2011Article
- Novel stem/progenitor cell population from murine tracheal submucosal gland ducts with multipotent regenerative potential.Stem cells (Dayton, Ohio) · 2011Article
- Branching morphogenesis of immortalized human bronchial epithelial cells in three-dimensional culture.Differentiation; research in biological diversityArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
To create a model system that investigates mechanisms resulting in hyperplasia and hypertrophy of respiratory tract submucosal glands, we developed an in vitro three-dimensional (3D) system wherein normal human bronchial epithelial (HBE) cells differentiated into glandular acini when grown on a basement membrane matrix. The differentiation of primary HBE cells into glandular acini was monitored temporally by light microscopy. Apoptosis-induced lumen formation was observed by immunofluorescence analysis. The acinar cells expressed and secreted MUC5B mucin (marker for glandular mucous cells) and lysozyme, lactoferrin, and zinc-α2-glycoprotein (markers for glandular serous cells) at Day 22. β-Tubulin IV, a marker for ciliated cells, was not detected. Expression of mucous and serous cell markers in HBE glandular acini demonstrated that HBE cells grown on a basement membrane matrix differentiated into acini that exhibit molecular characteristics of respiratory tract glandular acinar cells. Inhibition studies with neutralizing antibodies resulted in a marked decrease in size of the spheroids at Day 7, demonstrating that laminin (a major component of the basement membrane matrix), the cell surface receptor integrin α6, and the cell junction marker E-cadherin have functional roles in HBE acinar morphogenesis. No significant variability was detected in the average size of glandular acini formed by HBE cells from two normal individuals. These results demonstrated that this in vitro model system is reproducible, stable, and potentially useful for studies of glandular differentiation and hyperplasia.
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