Evidence map›Paper›PMID 20723536›Full record

ArticleAnalytical biochemistry2011

Targeted messenger RNA profiling of transfected breast cancer gene in a living cell.

D Nawarathna, R Chang, E Nelson, H Kumar Wickramasinghe

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Nanoneedle-Based Materials for Intracellular Studies.Advances in experimental medicine and biology · 2021
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

D NawarathnaDepartment of Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, USA.
R Chang
E Nelson
H Kumar Wickramasinghe

Funding

High throughput low cost DNA sequencing using probe tip arraysR01HG004431 · NHGRI · UNIVERSITY OF CALIFORNIA-IRVINE · PI WICKRAMASINGHE, HEMANTHA KUMAR · 2007 to 2009
$2.2M
Rapid DNA Sizing and Haplotyping Using Probe ElectophoresisR21HG004549 · NHGRI · UNIVERSITY OF CALIFORNIA-IRVINE · PI WICKRAMASINGHE, HEMANTHA KUMAR · 2008 to 2009
$420k
NHGRI NIH HHS HG-04431NHGRI NIH HHS HG-04549NHGRI NIH HHS R01 HG004431NHGRI NIH HHS R21 HG004549
6 · The paper itself

Abstract

Selective messenger RNA (mRNA) profiling of transfected breast cancer gene expression in a living cell is demonstrated. Atomic force microscope (AFM) probe tips are structurally modified to create a dielectrophoretic force that attracts mRNA molecules within the cell nucleus. The tip end is chemically modified to hybridize only to the target mRNA from a pool of molecules within the nucleus. We successfully combined this scheme with standard assay techniques to develop an assay technology that can be used for early disease detection and basic studies in cell biology.

Indexed as

AnimalsBreast NeoplasmsCell Line, TumorFemaleGene Expression RegulationGlyceraldehyde-3-Phosphate DehydrogenasesMicroscopy, Atomic ForceNucleic Acid HybridizationRatsReceptors, G-Protein-CoupledRNA, MessengerTransfectionGlyceraldehyde-3-Phosphate DehydrogenasesReceptors, G-Protein-CoupledRNA, MessengerUts2r protein, rat

Identifiers

PMID20723536
PMCPMC2980590

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.