ArticleVirology journal2010
Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- HPV E1 qPCR, a Low-Cost Alternative Assay to Roche Diagnostic Linear Array is Effective in Identifying Women at Risk for Developing Cervical Cancer.International journal of women's health · 2022Article
- Human Papillomavirus Oral Infection: Review of Methodological Aspects and Epidemiology.Pathogens (Basel, Switzerland) · 2021Review
- Detection of 14 High-Risk Human Papillomaviruses Using Digital LAMP Assays on a Self-Digitization Chip.Analytical chemistry · 2021Article
- Evidence for Missing Positive Results for Human Papilloma Virus 45 (HPV-45) and HPV-59 with the SPFJournal of clinical microbiology · 2020Article
- Viral Determinants in H5N1 Influenza A Virus Enable Productive Infection of HeLa Cells.Journal of virology · 2020Article
- Article
- Association of marijuana use with oral HPV infection and periodontitis among Hispanic adults: Implications for oral cancer prevention.Journal of periodontology · 2018Article
- CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.Science (New York, N.Y.) · 2018Article
- Whole-Genome Sequencing and Variant Analysis of Human Papillomavirus 16 Infections.Journal of virology · 2017Article
- Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: A developmental study.PLoS neglected tropical diseases · 2017Article
- Human papillomavirus infection in anal intraepithelial lesions from HIV infected Cuban men.Infectious agents and cancer · 2017Article
- Human papillomavirus infection in the oral cavity of HIV patients is not reduced by initiating antiretroviral therapy.AIDS (London, England) · 2016Observational
- The Oral HIV/AIDS Research Alliance Program: lessons learned and future directions.Oral diseases · 2016Article
- Prevalence of human papillomavirus in saliva of women with HPV genital lesions.Infectious agents and cancer · 2016Article
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- Presence of human polyomavirus 6 in mutation-specific BRAF inhibitor-induced epithelial proliferations.JAMA dermatology · 2014Article
- Analysis of human papillomavirus and herpes simplex virus genus -2 from patients with cervical cancer in isfahan, iran.Materia socio-medica · 2014Article
- High incidence of oncogenic HPV genotypes found in women from Southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Screening and detection of human papillomavirus (HPV) high-risk strains HPV16 and HPV18 in saliva samples from subjects under 18 years old in Nevada: a pilot study.BMC oral health · 2012Article
- Absence of human papillomavirus in patients with breast cancer in north-west China.Medical oncology (Northwood, London, England) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundHuman papillomaviruses (HPVs) remain a serious world health problem due to their association with anogenital/oral cancers and warts. While over 100 HPV types have been identified, a subset is associated with malignancy. HPV16 and 18 are the most prevalent oncogenic types, while HPV6 and 11 are most commonly responsible for anogenital warts. While other quantitative PCR (qPCR) assays detect oncogenic HPV, there is no single tube assay distinguishing the most frequent oncogenic types and the most common types found in warts.
resultsA Sybr Green-based qPCR assay was developed utilizing degenerate primers to the highly conserved HPV E1 theoretically detecting any HPV type. A single tube multiplex qPCR assay was also developed using type-specific primer pairs and TaqMan probes that allowed for detection and quantitation of HPV6,11,16,18. Each HPV type was detected over a range from 2 x 10(1) to 2 x 10(6)copies/reaction providing a reliable method of quantitating type-specific HPV in 140 anogenital/cutaneous/oral benign and malignant specimens. 35 oncogenic and low risk alpha genus HPV types were detected. Concordance was detected in previously typed specimens. Comparisons to the gold standard detected an overall sensitivity of 89% (95% CI: 77% - 96%) and specificity of 90% (95%CI: 52% - 98%).
conclusionThere was good agreement between the ability of the qPCR assays described here to identify HPV types in malignancies previously typed using standard methods. These novel qPCR assays will allow rapid detection and quantitation of HPVs to assess their role in viral pathogenesis.
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