ArticleAnalytical chemistry2010
Confocal imaging to quantify passive transport across biomimetic lipid membranes.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Assessing the mechanism of facilitated proton transport across GUVs trapped in a microfluidic device.Biophysical journal · 2024Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Permeation Studies across Symmetric and Asymmetric Membranes in Microdroplet Arrays.Analytical chemistry · 2021Article
- Hydrodynamic accumulation of small molecules and ions into cell-sized liposomes against a concentration gradient.Communications chemistry · 2020Article
- Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures.ChemistryOpen · 2018Article
- A synthetic enzyme built from DNA flips 10Nature communications · 2018Article
- Article
- Droplet-interface-bilayer assays in microfluidic passive networks.Scientific reports · 2015Article
- Visualization and quantification of transmembrane ion transport into giant unilamellar vesicles.Angewandte Chemie (International ed. in English) · 2015Article
- Hydrogel-assisted functional reconstitution of human P-glycoprotein (ABCB1) in giant liposomes.Biochimica et biophysica acta · 2015Article
- Low levels of lipid oxidation radically increase the passive permeability of lipid bilayers.Soft matter · 2015Article
- Lipid directed intrinsic membrane protein segregation.Journal of the American Chemical Society · 2013Article
- Optical stretching as a tool to investigate the mechanical properties of lipid bilayers.RSC advances · 2013Article
- Imaging molecular transport across lipid bilayers.Biophysical journal · 2011Article
- Microfluidic fabrication of asymmetric giant lipid vesicles.ACS applied materials & interfaces · 2011Article
- Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators.Applied physics letters · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability of a molecule to pass through the plasma membrane without the aid of any active cellular mechanisms is central to that molecule's pharmaceutical characteristics. Passive transport has been understood in the context of Overton's rule, which states that more lipophilic molecules cross membrane lipid bilayers more readily. Existing techniques for measuring passive transport lack reproducibility and are hampered by the presence of an unstirred layer (USL) that dominates transport across the bilayer. This report describes assays based on spinning-disk confocal microscopy (SDCM) of giant unilamellar vesicles (GUVs) that allow for the detailed investigation of passive transport processes and mechanisms. This approach allows the concentration field to be directly observed, allowing membrane permeability to be determined easily from the transient concentration profile data. A series of molecules of increasing hydrophilicity was constructed, and the transport of these molecules into GUVs was observed. The observed permeability trend is consistent with Overton's rule. However, the values measured depart from the simple partition-diffusion proportionality model of passive transport. This technique is easy to implement and has great promise as an approach to measure membrane transport. It is optimally suited to precise quantitative measurements of the dependence of passive transport on membrane properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.