ArticleBlood2010
Dose-dependent regulation of primitive erythroid maturation and identity by the transcription factor Eklf.
Article in Blood, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 38 citations in OpenAlex.
- KLF1 coordinates specialized transcriptional networks required to maintain the integrity of terminal erythropoiesis.Journal of cell science · 2025Article
- Erythroid Krüppel-Like Factor (KLF1): A Surprisingly Versatile Regulator of Erythroid Differentiation.Advances in experimental medicine and biology · 2024Review
- Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson's disease.Frontiers in aging neuroscience · 2024Article
- Review
- Hemoglobin switching in mice carrying theHaematologica · 2021Article
- KLF1/EKLF expression in acute leukemia is correlated with chromosomal abnormalities.Blood cells, molecules & diseases · 2020Article
- Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche.Biophysical reviews · 2019Review
- Genetic disarray follows mutant KLF1-E325K expression in a congenital dyserythropoietic anemia patient.Haematologica · 2019Article
- Survey and evaluation of mutations in the human KLF1 transcription unit.Scientific reports · 2018Article
- Deletion of Stk40 impairs definitive erythropoiesis in the mouse fetal liver.Cell death & disease · 2017Article
- Regulation of the embryonic erythropoietic niche: a future perspective.Blood research · 2017Review
- Development and differentiation of the erythroid lineage in mammals.Developmental and comparative immunology · 2016Review
- Interaction of the Macrophage and Primitive Erythroid Lineages in the Mammalian Embryo.Frontiers in immunology · 2016Review
- Extrinsic and intrinsic control by EKLF (KLF1) within a specialized erythroid niche.Development (Cambridge, England) · 2014Article
- Primitive and definitive erythropoiesis in mammals.Frontiers in physiology · 2014Review
- Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.Stem cells (Dayton, Ohio) · 2014Article
- Erythroid development in the mammalian embryo.Blood cells, molecules & diseases · 2013Review
- Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice.Genesis (New York, N.Y. : 2000) · 2013Article
- The modern primitives: applying new technological approaches to explore the biology of the earliest red blood cells.ISRN hematology · 2013Review
- Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing.Molecular and cellular biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
Abstract
The primitive erythroid (EryP) lineage is the first to differentiate during mammalian embryogenesis. Eklf/Klf1 is a transcriptional regulator that is essential for definitive erythropoiesis in the fetal liver. Dissection of the role(s) of Eklf within the EryP compartment has been confounded by the simultaneous presence of EryP and fetal liver-derived definitive erythroid (EryD) cells in the blood. To address this problem, we have distinguished EryP from their definitive counterparts by crossing Eklf(+/-) mutant and ε-globin::histone H2B-GFP transgenic mice. Eklf-deficient EryP exhibit membrane ruffling and a failure to acquire the typical discoidal erythroid shape but they can enucleate. Flow cytometric analyses of H2B-GFP(+) EryP revealed that Eklf heterozygosity results in the loss of Ter119 surface expression on EryP but not on EryD. Null mutation of Eklf resulted in abnormal expression of a range of surface proteins by EryP. In particular, several megakaryocyte markers were ectopically expressed by maturing Eklf-null EryP. Unexpectedly, the platelet tetraspanin CD9 was detected on nucleated wild-type EryP but not on mature EryD and thus provides a useful marker for purifying circulating EryP. We conclude that Eklf gene dosage is crucial for regulating the surface phenotype and molecular identity of maturing primitive erythroid cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.