ArticlePlant physiology2010
Stacking multiple transgenes at a selected genomic site via repeated recombinase-mediated DNA cassette exchanges.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Target lines for recombinase-mediated gene stacking in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Efficient Gene Stacking in Rice Using the GAANTRY System.Rice (New York, N.Y.) · 2021Article
- Recombinase-mediated integration of a multigene cassette in rice leads to stable expression and inheritance of the stacked locus.Plant direct · 2020Article
- Targeting Nitrogen Metabolism and Transport Processes to Improve Plant Nitrogen Use Efficiency.Frontiers in plant science · 2020Review
- High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.Plant biotechnology journal · 2019Article
- Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean.Frontiers in plant science · 2019Article
- Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- An Improved Variant of Soybean Type 1 Diacylglycerol Acyltransferase Increases the Oil Content and Decreases the Soluble Carbohydrate Content of Soybeans.Plant physiology · 2016Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion.BMC biotechnology · 2015Article
- Cas9-Guide RNA Directed Genome Editing in Soybean.Plant physiology · 2015Article
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
- Influence of carbon to nitrogen ratios on soybean somatic embryo (cv. Jack) growth and composition.Journal of experimental botany · 2013Article
- Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.Breeding science · 2012Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Enrichments of gene replacement events byMolecular breeding : new strategies in plant improvementArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinase-mediated DNA cassette exchange (RMCE) has been successfully used to insert transgenes at previously characterized genomic sites in plants. Following the same strategy, groups of transgenes can be stacked to the same site through multiple rounds of RMCE. A gene-silencing cassette, designed to simultaneously silence soybean (Glycine max) genes fatty acid ω-6 desaturase 2 (FAD2) and acyl-acyl carrier protein thioesterase 2 (FATB) to improve oleic acid content, was first inserted by RMCE at a precharacterized genomic site in soybean. Selected transgenic events were subsequently retransformed with the second DNA construct containing a Yarrowia lipolytica diacylglycerol acyltransferase gene (DGAT1) to increase oil content by the enhancement of triacylglycerol biosynthesis and three other genes, a Corynebacterium glutamicum dihydrodipicolinate synthetase gene (DHPS), a barley (Hordeum vulgare) high-lysine protein gene (BHL8), and a truncated soybean cysteine synthase gene (CGS), to improve the contents of the essential amino acids lysine and methionine. Molecular characterization confirmed that the second RMCE successfully stacked the four overexpression cassettes to the previously integrated FAD2-FATB gene-silencing cassette. Phenotypic analyses indicated that all the transgenes expressed expected phenotypes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.