Evidence map›Paper›PMID 20720026›Full record

ArticleJournal of clinical microbiology2010

Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens.

P Lewis White, Michael D Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J Peter Donnelly and 2 more

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 2 pooled it
4.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 89 citations in OpenAlex.

  1. Pooled it
  2. Guideline
  3. Review
  4. Review
  5. An Evaluation of the OLMJournal of fungi (Basel, Switzerland) · 2022
    Article
  6. Blood Aspergillus PCR: The Good, the Bad, and the Ugly.Journal of fungi (Basel, Switzerland) · 2020
    Review
  7. Review
  8. Article
  9. Molecular Diagnostic Testing for Aspergillus.Journal of clinical microbiology · 2016
    Review
  10. Article
  11. PCR Technology for Detection of Invasive Aspergillosis.Journal of fungi (Basel, Switzerland) · 2016
    Review
  12. Article
  13. Review
  14. Observational
  15. Clinical performance of a commercial real-time PCR assay for Aspergillus DNA detection in serum samples from high-risk patients: comparison with a galactomannan enzyme immunoassay.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015
    Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 8 institutions in 5 countries.

P Lewis WhitePublic Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom. lewis.white@wales.nhs.uk
Michael D Perry
Juergen Loeffler
Willem Melchers
Lena Klingspor
Stephane Bretagne
Elaine McCulloch
Manuel Cuenca-Estrella
Niklas Finnstrom
J Peter Donnelly
Rosemary A Barnes
European Aspergillus PCR Initiative
Public Health Wales · GBCardiff University · GBHôpitaux Universitaires Henri-Mondor · FRKarolinska University Hospital · SERadboud University Medical Center · NLRadboud University Nijmegen · NLRoyal Hospital for Children · GBUniversity of Würzburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

Indexed as

AspergillosisAspergillus fumigatusBloodDNA, FungalHumansMycologyPolymerase Chain ReactionSensitivity and SpecificityDNA, Fungal

Identifiers

PMID20720026
PMCPMC2953093
OpenAlexW2153483365

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.