ArticleThe Journal of biological chemistry2010
Activator-dependent p300 acetylation of chromatin in vitro: enhancement of transcription by disruption of repressive nucleosome-nucleosome interactions.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
- Roles of histone chaperone Nap1 and histone acetylation in regulating phase-separation of nucleosome arrays.Nature communications · 2025Article
- Group 3 medulloblastoma transcriptional networks collapse under domain specific EP300/CBP inhibition.Nature communications · 2024Article
- Molecular basis for SOX2-dependent regulation of super-enhancer activity.Nucleic acids research · 2023Article
- RP11-367G18.1 V2 enhances clear cell renal cell carcinoma progression via induction of epithelial-mesenchymal transition.Cancer medicine · 2023Article
- Super enhancers-Functional cores under the 3D genome.Cell proliferation · 2021Review
- Paradoxical activation of AMPK by glucose drives selective EP300 activity in colorectal cancer.PLoS biology · 2020Article
- Emerging roles of histone modifications and HDACs in RNA splicing.Nucleic acids research · 2019Review
- Milestones in transcription and chromatin published in theThe Journal of biological chemistry · 2019Review
- The 10-nm chromatin fiber and its relationship to interphase chromosome organization.Biochemical Society transactions · 2018Review
- Article
- Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays.Scientific reports · 2016Article
- Nucleosomal arrays self-assemble into supramolecular globular structures lacking 30-nm fibers.The EMBO journal · 2016Article
- The Influence of Ionic Environment and Histone Tails on Columnar Order of Nucleosome Core Particles.Biophysical journal · 2016Article
- Negative regulation of the p300-p53 interplay by DDX24.Oncogene · 2016Article
- Differential Roles for DUSP Family Members in Epithelial-to-Mesenchymal Transition and Cancer Stem Cell Regulation in Breast Cancer.PloS one · 2016Article
- In Vivo Acute on Chronic Ethanol Effects in Liver: A Mouse Model Exhibiting Exacerbated Injury, Altered Metabolic and Epigenetic Responses.Biomolecules · 2015Article
- How does HTLV-1 cause adult T-cell leukaemia/lymphoma (ATL)?Current opinion in virology · 2015Review
- BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia.Molecular cell · 2015Article
- Nucleosome-specific, time-dependent changes in histone modifications during activation of the early growth response 1 (Egr1) gene.The Journal of biological chemistry · 2015Article
- Epigenomics of macrophages.Immunological reviews · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Condensation of chromatin into higher order structures is mediated by intra- and interfiber nucleosome-nucleosome interactions. Our goals in this study were to determine the impact specific activator-dependent histone acetylation had on chromatin condensation and to ascertain whether acetylation-induced changes in chromatin condensation were related to changes in RNA polymerase II (RNAPII) activity. To accomplish this, an in vitro model system was constructed in which the purified transcriptional activators, Tax and phosphorylated CREB (cAMP-response element-binding protein), recruited the p300 histone acetyltransferase to nucleosomal templates containing the human T-cell leukemia virus type-1 promoter sequences. We find that activator-dependent p300 histone acetylation disrupted both inter- and intrafiber nucleosome-nucleosome interactions and simultaneously led to enhanced RNAPII transcription from the decondensed model chromatin. p300 histone acetyltransferase activity had two distinct components: non-targeted, ubiquitous activity in the absence of activators and activator-dependent activity targeted primarily to promoter-proximal nucleosomes. Mass spectrometry identified several unique p300 acetylation sites on nucleosomal histone H3 (H3K9, H3K27, H3K36, and H3K37). Collectively, our data have important implications for understanding both the mechanism of RNAPII transcriptional regulation by chromatin and the molecular determinants of higher order chromatin structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.