ArticleNucleic acids research2010
A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Article
- Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Hsp90 interaction networks in fungi-tools and techniques.FEMS yeast research · 2021Review
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- Article
- A High-ThroughputmSphere · 2018Article
- Generating genomic platforms to study Candida albicans pathogenesis.Nucleic acids research · 2018Article
- A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions inG3 (Bethesda, Md.) · 2017Article
- The Hog1 MAP Kinase Promotes the Recovery from Cell Cycle Arrest Induced by Hydrogen Peroxide inFrontiers in microbiology · 2016Article
- Libraries for two-hybrid screening of yeast and hyphal growth forms in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- A FACS-optimized screen identifies regulators of genome stability in Candida albicans.Eukaryotic cell · 2015Article
- Ascorbic acid inhibition of Candida albicans Hsp90-mediated morphogenesis occurs via the transcriptional regulator Upc2.Eukaryotic cell · 2014Article
- Role of the BaeSR two-component system in the regulation of Acinetobacter baumannii adeAB genes and its correlation with tigecycline susceptibility.BMC microbiology · 2014Article
- Ser or Leu: structural snapshots of mistranslation in Candida albicans.Frontiers in molecular biosciences · 2014Review
- An expanded genetic code in Candida albicans to study protein-protein interactions in vivo.Eukaryotic cell · 2013Article
- Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans.Microbiology (Reading, England) · 2013Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Prediction of phenotype-associated genes via a cellular network approach: a Candida albicans infection case study.PloS one · 2012Article
- Article
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3 authors.
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Abstract
The genetics of the most common human pathogenic fungus Candida albicans has several unique characteristics. Most notably, C. albicans does not follow the universal genetic code, by translating the CUG codon into serine instead of leucine. Consequently, the use of Saccharomyces cerevisiae as a host for yeast two-hybrid experiments with C. albicans proteins is limited due to erroneous translation caused by the aberrant codon usage of C. albicans. To circumvent the need for heterologous expression and codon optimalization of C. albicans genes we constructed a two-hybrid system with C. albicans itself as the host with components that are compatible for use in this organism. The functionality of this two-hybrid system was shown by successful interaction assays with the protein pairs Kis1-Snf4 and Ino4-Ino2. We further confirmed interactions between components of the filamentation/mating MAP kinase pathway, including the unsuspected interaction between the MAP kinases Cek2 and Cek1. We conclude that this system can be used to enhance our knowledge of protein-protein interactions in C. albicans.
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